CoVFlu-II One Step RT PCR Kit

Multiplex Detection of COVID-19 & Influenza A/B by Real-Time RT-PCR

The CoVFlu-II One Step RT PCR Kit is a CE-IVD molecular diagnostic assay designed for the qualitative detection of SARS-CoV-2 (COVID-19), Influenza A, and Influenza B in clinical samples.

This multiplex assay supports rapid differential diagnosis of viral respiratory infections, enabling timely clinical decisions, patient isolation measures, and outbreak surveillance through a single real-time RT-PCR workflow.

Product Overview


The CoVFlu-II One Step RT PCR Kit is an in vitro diagnostic (IVD) test based on advanced real-time RT-PCR technology for simultaneous detection of COVID-19 and Influenza A/B from a single patient sample.

The assay uses a single-tube multiplex design integrating viral RNA extraction (automated on compatible systems), Reverse transcription and Real-time PCR amplification within one streamlined workflow.

Why Multiplex Respiratory Testing Matters


COVID-19 and Influenza share overlapping symptoms, including:

Accurate differentiation is clinically critical because:

Multiplex RT-PCR enables rapid pathogen identification in a single assay, improving diagnostic efficiency and patient management.

Pathogens Detected


Viral Targets
  • SARS-CoV-2 (COVID-19)
  • Influenza A
  • Influenza B
Internal Control
  • Human RNaseP gene -
    Ensures RNA extraction quality and assay validity.

Applications


The kit is ideal for clinical molecular laboratories performing:

BCR-ABL Universal
  • COVID-19 RT-PCR testing
  • Influenza A/B detection
  • Differential diagnosis of viral respiratory infections
  • Seasonal flu surveillance
  • Outbreak monitoring programs
  • Hospital and emergency screening workflows

Key Features & Benefits


3-Pathogen Multiplex Detection

Simultaneous identification of COVID-19, Flu A, and Flu B in one assay.

Single-Tube Real-Time RT-PCR Design

Reduces handling steps and contamination risk.

Rapid Differential Diagnosis

Supports efficient triaging during respiratory infection seasons.

CE-IVD Validated Performance

Designed for professional clinical laboratory use.

Sample Types & Reporting Output


Sample Types

Validated for use with respiratory specimens, including:

  • Nasopharyngeal swabs
  • Oropharyngeal swabs
  • Samples should be transported at 2–8°C and processed within recommended timelines to maintain RNA integrity.
Reporting Output

The assay provides clear qualitative reporting, including:

  • Pathogen-specific Positive / Negative result
  • Ct values for each target
  • Amplification curves
  • Internal control validation
  • Exportable reports via USB or Bluetooth (system dependent)

Compatible System


OnePCR-Rapi-Q Ultra-Fast POC System

Workflow (OnePCR System):

OnePCR® workflow
  • Collect NP/OP swab sample
  • Load 200 µl sample into Cartridge-1
  • Reconstitute Cartridge-2 reagents
  • Insert cartridges into system
  • Automated RNA extraction + multiplex RT-PCR
  • Results displayed as pathogen-specific Positive/Negative with Ct curves

Storage & Stability


Ordering Information


Commercial Name Old Cat No. New Cat No. Pack Size
CoVFlu-II One Step RT PCR Kit G2M709821R 24 Tests

Why Choose the CoVFlu-II One Step RT PCR Kit?


If your lab requires a reliable COVID-19 and Influenza multiplex RT-PCR kit, this assay delivers:

  • simultaneous detection of SARS-CoV-2, Flu A & Flu B
  • single-tube multiplex workflow
  • internal control-validated results
  • automated extraction-to-result integration
  • suitability for hospital, reference lab, and surveillance testing
  • CE-IVD compliant respiratory molecular diagnostics

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