Arboviral infections such as Dengue, Zika, and Chikungunya are among the most pressing public health challenges in tropical and subtropical regions. Transmitted primarily by Aedes mosquitoes, these viruses frequently present with overlapping symptoms including fever, rash, joint pain, nausea, and fatigue, making clinical differentiation difficult. In severe cases, infection can progress to neuroinvasive disease, leading to encephalitis, meningitis, seizures, and long-term complications. As RNA viruses with high mutability, they pose additional diagnostic complexity, and traditional serological tests may lack sensitivity in early stages—underscoring the need for rapid, accurate molecular diagnostics capable of detecting and differentiating these pathogens simultaneously.
The Para2-Q Real-Time PCR Kit from Genes2Me is an innovative multiplex in vitro diagnostic assay designed for the qualitative detection of Dengue virus, Zika virus, and Chikungunya virus from whole blood, serum, and plasma. By combining advanced multiplex technology with robust quality controls, the kit enables faster clinical decision-making, improved patient outcomes, and stronger outbreak preparedness.
Genes2Me is a CE Marked molecular diagnostics company trusted by clinical laboratories across global markets. The Para2-Q Real-Time PCR Kit meets European conformity standards for in vitro diagnostic devices, validated to international benchmarks for accuracy, reliability, and safety.
The Para2-Q kit employs qRT-PCR with TaqMan probe chemistry in a streamlined single-tube format. Specific primers and fluorescent probes enable simultaneous detection and differentiation of three arboviruses, each assigned a dedicated fluorescence channel:
The Internal Control in the HEX channel verifies RNA quality and PCR efficiency across every run, eliminating false negatives arising from poor sample quality or inhibition. A positive control validates assay performance, while a negative control safeguards against contamination and workflow errors. Ready-to-use reagents and a simple protocol minimise hands-on time and reduce the potential for procedural errors.
Dengue, Zika, and Chikungunya share clinical presentations that make differential diagnosis unreliable without laboratory confirmation. Serological tests may lack sensitivity during the early viremic phase, and single-pathogen assays require multiple tests and additional turnaround time. As RNA viruses with high mutability, each pathogen demands sensitive and specific molecular targets. Multiplex RT-qPCR detection of virus-specific RNA provides the earliest confirmation of active infection, ahead of antibody seroconversion, and is critical for prompt, targeted clinical management. The four-channel design with a built-in internal control ensures that every negative result reflects a true absence of target viruses rather than a failed reaction.
Simultaneously detects and differentiates Dengue, Zika, and Chikungunya virus RNA in a single tube using dedicated fluorescence channels, reducing time and resource burden compared to individual assays.
The HEX-channel internal control verifies RNA quality and PCR efficiency in every run, ensuring confidence in every result and eliminating false negatives from poor sample quality or inhibition.
Safeguard against contamination and workflow errors, validating assay performance and reagent integrity in every run.
Reliable identification of viral RNA even at low viral loads, enabling confident diagnosis during early or subclinical infection.
Results available significantly faster than traditional serological methods, enabling clinicians to initiate timely and targeted patient management decisions.
Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480, supporting seamless integration into existing laboratory workflows.
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| Para2-Q Real-Time PCR Kit | G2M801521 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
© 2025 Genes2me. All rights reserved.
