Mycoplasma hominis is a bacterial pathogen belonging to the Mycoplasma genus, characterized by the absence of a cell wall. While it may exist as part of the normal genital microbiota in healthy individuals, under certain conditions it can become pathogenic and contribute to a range of urogenital infections. M. hominis has been associated with pelvic inflammatory disease (PID), bacterial vaginosis, urinary tract infections (UTIs), postpartum infections, and complications in immunocompromised patients.
The absence of a cell wall makes M. hominis inherently resistant to antibiotics that target cell wall synthesis, such as penicillins and cephalosporins, highlighting the importance of accurate pathogen identification for appropriate clinical management. Conventional culture methods are often laborious and time-consuming, whereas molecular diagnostic techniques offer rapid and highly sensitive detection.
The M. Hominis-Q Real-Time PCR Kit from Genes2Me is a molecular diagnostic assay designed for the qualitative detection of Mycoplasma hominis DNA in urine and genital swab specimens. Utilizing real-time PCR technology, the assay enables reliable identification of active infection, supporting clinicians in making timely treatment decisions and improving patient outcomes.
The M. Hominis-Q assay is based on real-time PCR technology and employs highly specific primers and fluorescent probes targeting conserved genomic regions of Mycoplasma hominis. The target-specific probe is detected in the Texas Red channel, while an integrated Internal Control is monitored in the Cy5 channel to verify sample quality, nucleic acid extraction efficiency, and PCR performance.
The assay is designed as a single-tube reaction, simplifying workflow while minimizing contamination risk. Positive and negative controls are incorporated into the testing process to ensure assay reliability and validate run performance. This combination of specificity, sensitivity, and internal quality control provides confidence in every reported result.
Clinical manifestations of M. hominis infection are often non-specific and may overlap with those caused by other sexually transmitted or urogenital pathogens. Furthermore, asymptomatic colonization can complicate diagnosis using conventional methods. Molecular detection offers a rapid and highly sensitive approach for identifying M. hominis DNA directly from clinical specimens.
Real-time PCR enables earlier and more accurate detection compared to culture-based methods, facilitating prompt therapeutic intervention and helping prevent complications associated with untreated infections. The high analytical sensitivity of molecular testing also makes it suitable for detecting low bacterial loads that may otherwise go unnoticed.
Targets Mycoplasma hominis DNA with highly specific primers and probes for accurate pathogen identification.
Cy5-channel Internal Control verifies extraction quality and PCR efficiency, reducing the risk of false-negative results.
Real-time PCR enables faster diagnosis compared to conventional culture methods.
Simplified assay setup minimizes hands-on time and reduces contamination risk.
Positive and negative controls validate assay performance in every run.
Ct values ≤35 are considered positive, enabling detection even at low target concentrations.
Stable performance under recommended storage conditions for improved inventory management.
Validated on leading real-time PCR systems including QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96.
M.Hominis-Q Real Time PCR specific amplification plot of M.hominis Positive Control (TEXAS RED Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| M. Hominis-Q Real-Time PCR Kit | G2M707221 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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