Herpes Simplex Virus (HSV) infections are among the most prevalent viral infections worldwide, caused by Herpes Simplex Virus Type 1 (HSV-1) and Type 2 (HSV-2), members of the Orthoherpesviridae family. HSV-1 is primarily associated with oral herpes infections, including cold sores and oral lesions, while HSV-2 is predominantly linked to genital herpes infections transmitted through sexual contact. Both viruses establish lifelong latent infections and may reactivate periodically, leading to recurrent symptomatic episodes and continued viral transmission.
Although many HSV infections remain asymptomatic or present with mild symptoms, active infections can cause painful blisters, ulcers, fever, swollen lymph nodes, and significant discomfort. In immunocompromised individuals, neonates, and pregnant women, HSV infections may lead to severe complications including disseminated disease, encephalitis, and neonatal herpes, making rapid and accurate diagnosis critically important.
The HSV-Q Real-Time PCR Test Kit for Herpes Simplex Virus 1 & 2 from Genes2Me is a highly sensitive and specific in vitro diagnostic assay designed for the qualitative detection and differentiation of HSV-1 and HSV-2 DNA in clinical samples using Real-Time PCR technology. The assay utilises type-specific primers and fluorescent hydrolysis probes targeting conserved genetic regions of HSV-1 and HSV-2, enabling accurate and simultaneous detection in a single reaction.
The HSV-Q assay is based on Real-Time PCR technology using highly specific primers and fluorescent probes designed to detect HSV-1 and HSV-2 DNA targets simultaneously. The assay specifically targets:
Amplified HSV-1 DNA is detected in the FAM channel, while HSV-2 amplification is detected in the Texas Red channel. An Internal Control targeting the human RNaseP gene is monitored in the Cy5 channel, ensuring sample adequacy and amplification integrity in every reaction. Ready-to-use reagents simplify workflow, reduce hands-on preparation time, and minimise the risk of contamination or procedural variability.
Clinical symptoms of HSV infection can overlap with other sexually transmitted infections and dermatological conditions, making symptom-based diagnosis unreliable. Serological testing may not accurately distinguish active from latent infection and may fail during early infection stages before antibody development.
Molecular detection through Real-Time PCR provides highly sensitive and specific identification of active HSV infection by directly detecting viral DNA. Simultaneous differentiation of HSV-1 and HSV-2 supports accurate diagnosis, patient counselling, infection management, and therapeutic decision-making. Rapid molecular diagnosis is particularly important for:
Detect and differentiates HSV-1 and HSV-2 in a single reaction using multiplex Real-Time PCR technology.
Type-specific primers and probes ensure precise identification of HSV-1 and HSV-2 targets with minimal cross-reactivity.
RNaseP Internal Control in the Cy5 channel validates sample quality, nucleic acid extraction, and amplification efficiency in every run.
Provides sensitive molecular detection with a Ct cut-off value of ≤35 for confident result interpretation.
Ensures assay integrity, workflow validation, and contamination monitoring across every PCR run.
Simultaneous detection of HSV-1, HSV-2, and Internal Control reduces turnaround time and reagent consumption.
Validated on multiple leading Real-Time PCR systems including QuantStudio™ 5, Bio-Rad CFX96, LightCycler® 480, and RapiCycler 96.
pre-optimised reagents reduce hands-on time, simplify assay setup, and minimise procedural variability.
Herpes Simplex Virus Real Time PCR specific amplification plot of HSV 1 Positive Control (FAM Channel) & HSV 2 Positive Control (Texas Red Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| HSV-Q Real-Time PCR Test Kit | G2M705221 | G610009-1 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
© 2025 Genes2me. All rights reserved.
