EGFR-Q Real-Time PCR Kit
EGFR-Q Real Time PCR specific amplification plot of EGFR positive sample (FAM channel) along with internal control (HEX channel).
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The EGFR-Q Real-Time PCR Kit from Genes2Me is an advanced in vitro diagnostic solution for the qualitative detection of EGFR somatic mutations in genomic DNA extracted from clinical samples, including fresh tissue and FFPE (formalin-fixed paraffin-embedded) tissue. Designed for use by trained laboratory professionals, this kit delivers high precision, rapid performance, and reliable results to enable confident, personalized treatment decisions in non-small cell lung cancer (NSCLC).
Why is EGFR Testing Critical?- Mutations in the Epidermal Growth Factor Receptor (EGFR) are strongly implicated in the development of non-small cell lung cancer (NSCLC), driving tumor progression, promoting angiogenesis, and contributing to resistance to apoptosis in lung malignancies. Lung cancer remains the leading cause of cancer-related deaths worldwide, with NSCLC accounting for 80% of cases.
Clinical studies have shown that the effectiveness of EGFR tyrosine kinase inhibitors (TKIs) is directly linked to EGFR mutation status. By identifying mutations in exons 18–21 of the EGFR gene - particularly 19del (45% prevalence) and L858R (40 - 45% prevalence)- this kit empowers clinicians to select the right therapy and improve patient survival.
Detects clinically important EGFR mutations including L858R, T790M, G719X, Exon 19 deletions (19del), L861Q, Exon 20 insertions (3Ins20), and S768I, delivering a complete mutation profile to guide targeted therapy decisions.
Capable of detecting mutations at 0.25–0.5% allele frequency, ensuring reliable results even from challenging FFPE samples.
Continuously monitors sample quality, reagent integrity, and assay workflow to minimize false-negative results.
Optimized real-time PCR workflow provides precise mutation results within hours, supporting timely clinical decision-making.
Features UDG enzyme technology to prevent carryover contamination, ensuring assay reliability.
Validated with leading PCR systems including QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96.
EGFR-Q Real Time PCR specific amplification plot of EGFR positive sample (FAM channel) along with internal control (HEX channel).
Fold 80 values for blood samples ranged from 1.23–1.35, showing highly uniform coverage with minimal sequencing bias supporting accurate and dependable variant detection.
Coverage patterns for key cancer genes (BRCA1, PTEN, MSH2, MSH6, RET) show strong consistency, with mean (solid) and median (dashed) values closely aligned.
Download useful documents and technical information of EGFR-Q.
| Commercial Name | Cat No. | Pack Size |
|---|---|---|
| EGFR-Q Real-Time PCR Kit | G2M708121 | 25 T |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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