Overview

The BCR-ABL Quantitative Real-Time PCR Kit is a highly reliable in vitro diagnostic assay designed for the quantitative detection of BCR-ABL1 fusion transcripts in RNA extracted from blood and bone marrow samples. The BCR-ABL1 gene fusion is commonly known as the Philadelphia chromosome abnormality, which is the hallmark of Chronic Myeloid Leukemia (CML) and a subset of Acute Lymphoblastic Leukemia (ALL) cases. Its detection confirms diagnosis and plays a pivotal role in treatment planning and disease monitoring. Quantifying the expression levels of this fusion gene also enables clinicians to evaluate treatment response and minimal residual disease, ensuring timely clinical interventions for better patient outcomes.

The BCR-ABL Quantitative Real-Time PCR kit incorporates specific primers and fluorescent probes for detecting the major BCR-ABL1 transcripts (p210, e14a2 and e13a2). Using FAM-labeled probes for the target fusion gene and Texas Red-labeled probes for the internal control (ABL1), the assay ensures high specificity, sensitivity, and reliability in every test. The inclusion of five quantification standards allows for precise standard curve generation, enabling accurate quantification of target transcripts and normalization to internal controls. The BCR-ABL Quantitative RT-PCR Kit is validated on widely used real-time PCR platforms, including QuantStudio™ 5 (Thermo Fisher), Bio-Rad CFX96, Roche LightCycler® 480, and RapiCycler 96 (Genes2Me). With a streamlined workflow, stable reagents, and optimized assay conditions, the kit offers unmatched consistency and convenience for clinical laboratories.

Powered by advanced real-time reverse-transcription PCR (RT-PCR) technology, this kit provides precise, accurate, and reproducible results that are critical for the diagnosis and monitoring of CML and certain cases of ALL.

Key features & highlights

High Sensitivity, Specificity, and Accuracy

Uses custom primers and fluorescent probes (FAM for TEL-AML1 and HEX for the internal control) to ensure precise detection, with reliable results demonstrated by detection of TEL-AML1 fusion gene with Ct ≤ 35.

Built-in Internal Control for Reliability

RNase P internal control validates sample quality and workflow accuracy, minimizing false negatives and guaranteeing dependable outcomes.

Efficient Multiplex Real-Time PCR

Enables simultaneous detection of multiple targets in a single reaction, enhancing workflow efficiency, conserving reagents, and supporting rapid turnaround.

Broad Platform Compatibility

Optimized for major real-time PCR systems including QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and RapiCycler 96, ensuring seamless integration into diverse laboratory setups.

Streamlined Workflow with Fast, Reproducible Results

Designed to deliver timely, consistent, and clinically actionable data to support critical decision-making in leukemia diagnostics.

Robust Stability and Long Shelf Life

Features optimized reagents and a 12-month stability profile, ensuring consistent performance and reliable outcomes across varying clinical laboratory conditions.

Key Features

Accurate Quantification: Reliable detection and measurement of major BCR-ABL1 transcripts for leukemia diagnosis and monitoring.
Built-in Internal Control: Validates test integrity and minimizes false-negative results.
High Sensitivity & Specificity: Detects low transcript levels, enabling effective MRD monitoring.
Standardized Workflow: Includes multiple quantification standards for robust curve generation.
Rapid Turnaround: Provides fast, reproducible results to support timely treatment decisions.
Broad Compatibility: Validated on leading PCR platforms used in clinical diagnostics.
Long Shelf Life: Stable reagents ensure consistent performance across the kit’s 12-month storage duration.

Designed for trained laboratory professionals, the BCR-ABL Quantitative Real-Time PCR Kit is a powerful diagnostic and monitoring tool for leukemia management. With its combination of precision, sensitivity, and ease of use, it empowers clinicians and laboratories to deliver accurate, timely, and actionable insights in the fight against CML and ALL.

Performance data

When the Ct value of the sample to be tested is outside the reference range and there is a typical S-shaped amplification curve, the test result is positive;
When the Ct value of the sample to be tested is within the reference range, or the Ct value is outside the reference range but there is no typical S-shaped amplification curve, the test result is negative.

hannel 1 Slope: -3.392 Intercept: 39.372 Correlation: -0.998 Efficiency3: 97.1430 Channel 3 Slope: -3.240 Intercept: 38.672 Correlation: -0.999 Efficiency2: 103.52

Commercial Name	Cat No.	Pack Size
BCR-ABL Quantitative Real Time PCR Kit	G2M802821	50 T

Commercial Name

Cat No.

Pack Size

BCR-ABL Quantitative Real Time PCR Kit

G2M802821

50 T

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

All products listed in the catalogue are the products of Genes2Me Private Limited. Apart from that, all other product names, trademarks and logos wherever used in the catalogue are the property of their respective owners.

Overview