Overview

The JAK2-Q Real-Time PCR Kit from Genes2Me is a high-performance molecular diagnostic solution for the rapid and accurate detection of the JAK2 V617F (1849 G>T) mutation from clinical samples. This mutation is an essential biomarker in the diagnosis of myeloproliferative neoplasms (MPNs), including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF).

With its optimized assay design, stringent quality checks, and international standard compliance, the JAK2-Q kit delivers high sensitivity, non-invasive testing, and fast turnaround times - empowering laboratories and clinicians with reliable results for early detection, treatment guidance, and disease monitoring. Combining speed, precision, and convenience, the JAK2-Q kit enhances laboratory efficiency and supports improved patient outcomes, making it a trusted choice for advanced diagnostics.

Key features & highlights

Exceptional Sensitivity & Specificity

Detects the JAK2 V617F mutation at very low allelic frequencies, ensuring highly accurate and reliable results with every test.

Proven Analytical Performance

Demonstrates a limit of detection of 0.3%, high precision (CV < 4.5%), and excellent clinical accuracy (≥96% sensitivity and ≥98% specificity).

Dual Detection Channels

Utilizes distinct fluorescent probes for wild-type alleles (FAM channel) and mutant alleles (HEX channel), providing robust internal control and minimizing false-negative results.

Non-Invasive Testing

Requires only a standard blood sample, offering a convenient and patient-friendly alternative to invasive diagnostic procedures.

Fast Turnaround Time

Delivers results within hours, enabling rapid clinical decision-making compared to conventional methods such as bone marrow biopsy or cytogenetic testing.

Seamless Instrument Compatibility

Optimized for major real-time PCR platforms including QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96.

Applications

Accurate Diagnosis of MPNs:Detects the JAK2 V617F mutation, which is found in nearly all polycythemia vera cases and a significant number of ET and PMF patients, supporting precise clinical diagnosis.
Early Patient Stratification:Enables timely identification of mutation-positive patients, helping clinicians classify disease severity and guide appropriate management.
Personalized Treatment Planning:Provides rapid and reliable molecular insights that allow clinicians to tailor treatment strategies specific to the patient’s genetic profile.
Improved Long-Term Patient Outcomes:Supports earlier intervention and targeted therapy, contributing to better overall disease management and enhanced patient care.

Performance data

When the Ct value of the sample to be tested is outside the reference range and there is a typical S-shaped amplification curve, the test result is positive;
When the Ct value of the sample to be tested is within the reference range, or the Ct value is outside the reference range but there is no typical S-shaped amplification curve, the test result is negative.

Commercial Name	Cat No.	Pack Size
Jak2-Q Real Time PCR Kit	G2M802621	50 T

Commercial Name

Cat No.

Pack Size

Jak2-Q Real Time PCR Kit

G2M802621

50 T

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

All products listed in the catalogue are the products of Genes2Me Private Limited. Apart from that, all other product names, trademarks and logos wherever used in the catalogue are the property of their respective owners.

Overview