MDR-Q Real-Time PCR Kit

Qualitative Detection of Antimicrobial Resistance (AMR) Genes by Real-Time PCR

The MDR-Q Real-Time PCR Kit is a multiplex molecular assay designed for the qualitative detection of antimicrobial resistance (AMR) genes in human clinical samples using real-time PCR technology. The assay enables rapid identification of key resistance markers to support informed antimicrobial therapy decisions

At a Glance

Target: 18 antimicrobial resistance genes
Technology: Multiplex Real-Time PCR (probe-based)
Sample Type: Human clinical samples (whole blood)
Detection Type: Qualitative
Application: AMR gene detection

Product Overview

The assay is based on real-time PCR for the detection of resistance genes associated with Carbapenem, Vancomycin, Methicillin, Colistin, ESBLs, AmpC β-lactamase, Trimethoprim/Sulfamethoxazole (SXT), and PMQR resistance.

The kit consists of five reaction tubes, each targeting a specific group of resistance genes using fluorescent probes across FAM, HEX, Texas Red, and Cy5 channels. Tube 5 includes an internal control to validate assay performance.

How the Assay Works

Sample Collection & Nucleic Acid Extraction: DNA is extracted from clinical samples
Reaction Setup : DNA is added to tube-specific primer-probe mixes
PCR Amplification: Target resistance genes are amplified
Fluorescent Detection: Signals detected across FAM, HEX, Texas Red, Cy5
Result Interpretation: Based on gene-specific amplification patterns

Key Features & Benefits

Detection of 18 AMR genes
Multiplex assay design
Probe-based detection for specificity
Internal control for validation
Comprehensive resistance profiling

Why AMR Detection Matters

Antimicrobial resistance (AMR) reduces the effectiveness of antibiotics and complicates infection management. Rapid detection of resistance genes enables accurate antimicrobial selection and improves patient outcomes.

Targets Detected

Tube 1 – Carbapenem Resistance

VIM (FAM)
OXA-48 (HEX)
IMP (Texas Red)
NDM (Cy5)

Tube 2 – Methicillin, ESBL, Vancomycin, Colistin

mecA (FAM)
CTX-M (HEX)
vanA (Texas Red)
MCR-1 (Cy5)

Tube 3 – ESBL & AmpC

TEM (FAM)
vanB (HEX)
SHV (Texas Red)
citM (Cy5)

Tube 4 – SXT & PMQR

dfrA1 (FAM)
dfrA5 (HEX)
sul2 (Texas Red)
aac6’-Ib-cr (Cy5)

Tube 5 – Carbapenem + Internal Control

KPC (FAM)
OXA-23 (HEX)
Internal Control (Cy5)

Sample Type & Reporting Output

Sample Types

Whole blood

Reporting Output

Qualitative detection of AMR genes

Analytical Performance

Analytical Sensitivity:

100%

Analytical Specificity:

100%

Trueness (Bias):

98%

Accuracy:

99%

Limit of Detection (LoD):

100 copies/ml

Measuring Range:

10 – 10⁹ copies/reaction

Linearity:

R² = 0.999

Cut-off:

Ct ≤ 35

Cross-reactivity:

Not observed (Chlamydia pneumoniae, Mycoplasma pneumoniae, Bordetella pertussis, Legionella pneumophila)

Clinical Sensitivity:

98%

Clinical Specificity:

99%

Workflow Advantage

Simultaneous detection of multiple resistance genes

Reduced number of reactions via multiplex tubes

Faster turnaround compared to conventional AST methods

Supports high-throughput laboratory workflows

Designed for Laboratory Efficiency

The assay is optimized for streamlined workflow:

Pre-aliquoted primer-probe mixes (5-tube format)
Standardized reaction setup
Compatible with multiple real-time PCR platforms
Closed-tube detection reduces contamination risk

Storage and Stability

Shelf life: 12 months from date of manufacture
Storage: -20°C (shipped at -30°C to -10°C)
Avoid repeated freeze-thaw cycles (>6 times)
Protect from direct sunlight

Applications & Use Scenarios

The kit is intended for use in clinical and research laboratories for:

Detection of antimicrobial resistance genes
Infection management support
Molecular AMR surveillance
Clinical microbiology workflows

Ordering Information

Commercial Name	Old Cat No.	New Cat No.	Pack Size
MDR-Q Real-Time PCR Kit	G2M707521		50 Tests

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