Y. FEVER-Q Real-Time PCR Kit

Product Overview

The Y. FEVER-Q Real-Time PCR Kit is an in vitro diagnostic (IVD) assay based on advanced Real-Time Reverse Transcription PCR (RT-PCR) technology. The assay performs automated nucleic acid extraction and amplification using the OnePCR Ultra-Fast POC Rapid RT-PCR System.

The kit detects:

Yellow Fever Virus (YFV) RNA
Internal Control (IC) for assay validation and quality assurance

The assay utilizes a single-tube multiplex Real-Time RT-PCR format to accurately detect Yellow Fever Virus RNA in an automated sample-to-result workflow.

Why Yellow Fever Testing Matters

Yellow Fever is a mosquito-borne viral disease caused by the Yellow Fever Virus (YFV), a positive-sense single-stranded RNA virus belonging to the Flavivirus genus. The disease is primarily transmitted through the bites of infected Aedes aegypti and other mosquito species and remains endemic in many tropical regions of Africa and South America.

Yellow Fever can present in different clinical forms, including Urban, Intermediate, and Jungle (Sylvatic) transmission cycles. Following an incubation period, patients commonly develop:

Sudden onset of fever
Severe headache
Muscle and joint pain
Flushing
Loss of appetite
Nausea and vomiting
Jaundice

Early molecular diagnosis is essential for prompt patient management, outbreak surveillance, infection control, and public health interventions. The Y. FEVER-Q Real-Time PCR Kit helps laboratories to:

Rapidly detect Yellow Fever Virus RNA
Support early clinical diagnosis
Facilitate timely patient management
Improve diagnostic confidence in suspected Yellow Fever cases
Strengthen laboratory-based surveillance of arboviral diseases

Targets Detected

Qualitative Targets

Yellow Fever Virus (YFV) RNA
The assay specifically targets the conserved Polyprotein gene region of the Yellow Fever Virus genome for highly specific molecular detection.

Internal Control

Internal Control (IC)
Ensures successful RNA extraction, assay integrity, sample adequacy, and reliable amplification for every clinical sample.

Applications

The kit is ideal for clinical molecular laboratories performing:

Yellow Fever molecular detection
Early diagnosis of acute Yellow Fever infection
Confirmation of suspected Yellow Fever cases
Outbreak investigation and epidemiological surveillance
Integration into infectious disease molecular diagnostic workflows

Key Features & Benefits

Automated Sample-to-Result Workflow

Integrated with the OnePCR Ultra-Fast POC Rapid RT-PCR System, providing fully automated RNA extraction, and Real- Time PCR amplification with minimal hands-on time.

Sensitive and Specific Detection

Utilizes highly specific primer-probe chemistry targeting the conserved Polyprotein gene of Yellow Fever Virus.

Single-Tube Multiplex Assay

Employs a multiplex RT-PCR assay that simultaneously detects: Yellow Fever Virus RNA Internal Control within a single automated reaction.

Internal Control for Quality Assurance

A built-in Internal Control continuously monitors the complete testing process from nucleic acid extraction through amplification, ensuring assay reliability and result accuracy.

Optimized for Routine Clinical Testing

Designed for routine molecular diagnostics in hospitals, clinical laboratories, infectious disease centers, and public health laboratories performing arboviral surveillance.

Sample Types & Reporting Output

Sample Types

Validated for use with:

Whole Blood (EDTA)
Serum
Plasma

Reporting Output

The test provides clear and actionable laboratory results, including:

Positive / Negative interpretation
Ct values
Amplification curves
Internal Control status
Export of reports through USB or Bluetooth using the OnePCR System

Compatible System

OnePCR Ultra-Fast POC Rapid RT-PCR System

The Y. FEVER-Q Real-Time PCR Kit is specifically designed for seamless integration with the OnePCR platform, enabling automated nucleic acid extraction, Real-Time RT-PCR amplification, and integrated result reporting through a rapid sample-to-result workflow.

Collect whole blood, serum, or plasma sample.
Load 200 µL of the patient sample into Cartridge-1.
Add 10 µL Reconstitution Buffer into Cartridge-2.
Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast RT-PCR System.
Results are displayed as Positive or Negative together with Ct values and amplification curves.
Export reports through USB or Bluetooth for documentation and review.

Storage & Stability

Store at 2°C – 8°C
Ship at 10°C–30°C
Shelf-life: 12 months
Stable under recommended storage and transportation conditions
Protect from direct sunlight

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Y. FEVER-Q Real-Time PCR Kit	G2M801821R		24 Tests

Why Choose the Y. FEVER-Q Real-Time PCR Kit?

If your laboratory requires a reliable molecular assay for rapid detection of Yellow Fever Virus infection, the Y. FEVER-Q Real-Time PCR Kit delivers:

Automated RNA extraction and Real-Time RT-PCR workflow
Highly sensitive and specific detection of Yellow Fever Virus RNA
Polyprotein gene-targeted assay design
Integrated Internal Control for assay validation and quality assurance
Seamless integration with the OnePCR Ultra-Fast POC Rapid RT-PCR System
Suitable for routine molecular diagnostics, outbreak surveillance, and infectious disease testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Qualitative Detection of Yellow Fever Virus (YFV) RNA

Product Overview

Why Yellow Fever Testing Matters