W. Nile-Q Real-Time PCR Kit- Genes2Me | IVD RT-PCR Testing Kits

Product Overview

The W. Nile-Q Real-Time PCR Kit is an in vitro diagnostic (IVD) assay based on advanced Real-Time PCR technology. The assay performs automated nucleic acid extraction and amplification using the OnePCR Ultra-Fast POC RT PCR System.

The kit detects:

West Nile Virus RNA
Human RNaseP Internal Control (IC) for assay validation and quality assurance

The assay utilizes a single-tube multiplex Real-Time PCR format to accurately detect West Nile Virus RNA in an automated sample-to-result workflow.

Why West Nile Virus Testing Matters

West Nile Virus (WNV) is a mosquito-borne flavivirus that infects humans, birds, horses, and other mammals. While most infections remain asymptomatic, approximately 20% of infected individuals develop fever, headache, body aches, fatigue, and other flu-like symptoms. In a small proportion of patients, particularly older adults and immunocompromised individuals, the infection can progress to severe neuroinvasive disease, including encephalitis or meningitis, which may result in long-term neurological complications or death.

Early laboratory diagnosis is critical because:

The clinical presentation often resembles other viral febrile illnesses, making accurate differentiation challenging.
The clinical presentation often resembles other viral febrile illnesses, making accurate differentiation challenging.
Molecular detection using Real-Time PCR enables rapid, highly sensitive, and specific identification of West Nile Virus RNA during the early phase of infection, supporting timely clinical decision-making and patient management.

The W. Nile-Q Real-Time PCR Kit helps laboratories to:

rapidly detect West Nile Virus RNA
support early clinical diagnosis
facilitate timely patient management
improve diagnostic confidence in suspected WNV infections

Applications

The kit is ideal for clinical molecular laboratories performing:

West Nile Virus molecular detection
Early molecular diagnosis of WNV infection
Routine arboviral molecular testing
Infectious disease molecular diagnostic workflows

Key Features & Benefits

Automated Sample-to-Result Workflow

OnePCR Ultra-Fast POC RT PCR System for automated RNA extraction, amplification, with minimal hands-on time.

Sensitive and Specific Detection

for highly sensitive and specific detection of West Nile Virus RNA, it Utilizes target-specific primers and fluorescent probe-based chemistry.

Single-Tube Multiplex Assay

Simultaneously detects West Nile Virus RNA and the Human RNaseP Internal Control in a single multiplex Real-Time PCR reaction.

Built-in Internal Control

Human RNaseP Internal Control verifies successful nucleic acid extraction, sample quality, and assay performance.

Sample Types & Reporting Output

Sample Types

Validated for use with:

Whole blood (EDTA)
Cerebrospinal Fluid (CSF)

Reporting Output

The test provides clear and actionable laboratory results, including:

· Positive / Negative interpretation
Ct values
Amplification curves
Human RNaseP Internal Control status
Result export options (USB or Bluetooth via OnePCR System)

Compatible System

OnePCR Ultra-Fast RT PCR System

Designed for automated RNA extraction, Real-Time PCR amplification, and integrated sample-to-result workflow.

Collect whole blood or cerebrospinal fluid (CSF) specimen.
Load 200 µL of the sample into Cartridge-1.
Reconstitute Cartridge-2 by adding 10 µL Reconstitution Buffer.
Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast RT PCR System.
Perform fully automated RNA extraction and Real-Time PCR amplification.
Results displayed with Ct values, amplification curves, and Positive/Negative interpretation.

Storage & Stability

Store at 2°C – 8°C
Ship at 10°C–30°C
Shelf-life: 12 months
Stable under recommended storage and transportation conditions
Protect from direct sunlight

Commercial Name	Old Cat No.	New Cat No.	Pack Size
W. Nile-Q Real-Time PCR Kit	G2M802321R	G610042-3	24 Tests

Why Choose the W. Nile-Q Real-Time PCR Kit?

If your laboratory requires a reliable molecular assay for rapid detection of West Nile Virus RNA, the W. Nile-Q Real-Time PCR Kit delivers:

Automated RNA extraction and Real-Time PCR workflow
Highly sensitive and specific probe-based detection
Built-in Human RNaseP Internal Control for assay validation
Minimal hands-on time with an integrated sample-to-result workflow
Suitability for routine West Nile Virus molecular diagnostics
Infectious disease molecular testing workflows

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

W. Nile-Q Real-Time PCR Kit

Qualitative Detection of West Nile Virus RNA

Product Overview

Why West Nile Virus Testing Matters

Targets Detected

Qualitative Targets

Internal Control

Applications