Para2-Q Real Time PCR Kit | IVD RT-PCR Testing Kit

Product Overview

The PARA2-Q Real-Time PCR Kit is an advanced multiplex Real-Time RT-PCR assay for the qualitative detection of Dengue, Chikungunya, and Zika viruses from peripheral blood, plasma, and serum samples.

The assay is specifically optimized for the OnePCR Ultra-Fast POC Rapid RT-PCR System, integrating automated RNA extraction, and Real- Time PCR amplification into a seamless sample-to-result workflow with minimal manual intervention.

The kit detects:

Dengue Virus (DENV) RNA
Chikungunya Virus (CHIKV) RNA
Zika Virus (ZIKV) RNA
Internal Control (IC) for assay validation and quality assurance

The single-tube multiplex Real-Time RT-PCR design allows simultaneous detection of all three pathogens in a single reaction while continuously monitoring assay performance through an internal control, ensuring reliable and reproducible results.

Why PARA2-Q Testing Matters

Mosquito-borne viral infections continue to pose a significant public health challenge worldwide, particularly in tropical and subtropical regions. Dengue, Chikungunya, and Zika viruses are transmitted primarily by Aedes aegypti and Aedes albopictus mosquitoes and often present with similar clinical symptoms, including fever, rash, headache, muscle pain, and severe joint pain. This overlap makes clinical diagnosis difficult without laboratory confirmation.

Early and accurate molecular diagnosis is therefore essential to differentiate these infections, and initiate appropriate patient management.

The PARA2-Q Real-Time PCR Kit enables laboratories to:

Rapidly detect Dengue, Chikungunya, and Zika viruses
Differentiate three clinically similar infections in a single test
Support early clinical diagnosis
Improve patient management and treatment decisions
Strengthen outbreak surveillance and infectious disease monitoring

Targets Detected

Qualitative Targets

Dengue Virus (DENV) RNA
Chikungunya Virus (CHIKV) RNA
Zika Virus (ZIKV) RNA

Internal Control (IC)

Internal Control
The integrated Internal Control monitors RNA extraction efficiency, verifies assay integrity, confirms successful amplification

Applications

The PARA2-Q Real-Time PCR Kit is ideal for:

Molecular diagnosis of Dengue infection, Chikungunya infection, and Zika infection
Routine tropical fever molecular testing
Outbreak investigation and surveillance
Public health laboratories
Hospital and clinical molecular diagnostic laboratories

Key Features & Benefits

Automated Sample-to-Result Workflow

Designed exclusively for the OnePCR Ultra-Fast POC Rapid RT-PCR System, the assay integrates automated RNA extraction, and Real-Time RT-PCR amplification, into a single workflow.

Simultaneous Multiplex Detection

The assay employs a single-tube multiplex Real-Time RT-PCR format capable of detecting Dengue, Chikungunya, and Zika viruses simultaneously.

Highly Sensitive and Specific Detection

Target-specific primers and fluorescent hydrolysis probes provide highly sensitive and specific detection of viral RNA.

Internal Control for Quality Assurance

A built-in Internal Control validates every stage of testing, including RNA extraction and PCR amplification, minimizing the risk of false-negative results.

Optimized for Routine Clinical Testing

The assay is designed for routine molecular diagnostics in clinical laboratories, hospitals, emergency departments, and decentralized healthcare settings requiring rapid detection of tropical fever pathogens.

Sample Types & Reporting Output

Sample Types

Validated for use with:

Peripheral Whole Blood (EDTA)
Plasma
Serum

Reporting Output

The OnePCR software provides clear and comprehensive laboratory reports, including:

Positive / Negative interpretation
Individual detection of Dengue, Chikungunya, and Zika viruses
Ct values
Amplification curves
Internal Control status
Export of reports via USB or Bluetooth

Compatible System

OnePCR Ultra-Fast POC Rapid RT-PCR System

The PARA2-Q Real-Time PCR Kit is specifically designed for use with the OnePCR Ultra-Fast POC Rapid RT-PCR System (Genes2Me), which automates RNA extraction, and Real-Time RT-PCR amplification.

Collect peripheral whole blood, plasma, or serum sample.
Add 200 µL of the sample into Cartridge-1.
Reconstitute Cartridge-2 by adding 10 µL of Reconstitution Buffer.
Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast POC Rapid RT-PCR System.
The instrument automatically performs RNA extraction and multiplex Real-Time RT-PCR amplification.
Results are displayed as Positive or Negative for Dengue, Chikungunya, and Zika viruses along with Ct values, amplification curves, and Internal Control status.

Commercial Name	Old Cat No.	New Cat No.	Pack Size
PARA2-Q Real-Time PCR Kit	G2M801521R	G610041-3	24 Tests

Why Choose the PARA2-Q Real-Time PCR Kit?

If your laboratory requires a reliable molecular assay for rapid detection of malaria parasite DNA, the PARA2-Q Real-Time PCR Kit delivers:

Fully automated RNA extraction and Real-Time RT-PCR workflow
Simultaneous detection of Dengue, Chikungunya, and Zika viruses in a single reaction
Highly sensitive and specific multiplex detection
Integrated Internal Control for assay validation
Rapid and reliable molecular diagnosis for tropical fever pathogens
Standardized workflow that minimizes contamination risk
Seamless integration with the OnePCR Ultra-Fast POC Rapid RT-PCR System

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

PARA2-Q Real-Time PCR Kit

Qualitative Detection of Dengue Virus, Chikungunya Virus, and Zika Virus

Product Overview

Why PARA2-Q Testing Matters