J. encephalitis- Q Real-Time PCR Kit

Qualitative Detection of Japanese Encephalitis Virus (JEV) RNA

The J. ENCEPHALITIS- Q Real-Time PCR Kit is an in vitro diagnostic (IVD) molecular test designed for the qualitative detection of Japanese encephalitis virus (JEV) RNA from human clinical samples.

This assay enables rapid and accurate molecular detection of Japanese encephalitis virus through a standardized, automated Real-Time RT-PCR workflow, supporting timely diagnosis and appropriate clinical management of suspected Japanese encephalitis infections.

Product Overview

The J. ENCEPHALITIS- Q Real-Time PCR Kit is an in vitro diagnostic (IVD) assay based on advanced Real-Time RT-PCR technology. The assay performs automated nucleic acid extraction and amplification using the OnePCR Ultra-Fast RT PCR System, providing a streamlined sample-to-result workflow with minimal hands-on time.

The kit detects:

  • Japanese encephalitis virus (JEV) RNA
  • Human RNaseP Internal Control (IC) for assay validation and quality assurance

The assay utilizes a single-tube multiplex Real-Time RT-PCR format to accurately detect JEV RNA while simultaneously monitoring assay integrity through amplification of the Human RNaseP internal control. This integrated approach ensures reliable extraction, amplification, and result interpretation within an automated workflow.

j. encephalia-Q Real-Time PCR Kit

Why Japanese Encephalitis Testing Matters

Japanese encephalitis (JE) is a mosquito-borne viral disease caused by the Japanese encephalitis virus, a member of the Flavivirus genus. The virus is transmitted primarily through the bite of infected Culex mosquitoes, particularly Culex tritaeniorhynchus, with pigs and wading birds serving as important natural reservoirs.

Although the majority of infected individuals remain asymptomatic or develop only mild febrile illness, approximately 1 in 250 infections progresses to severe neurological disease, resulting in encephalitis characterized by high fever, seizures, neck stiffness, altered consciousness, paralysis, and other neurological complications. Severe disease is associated with significant mortality and long-term neurological sequelae among survivors. Therefore, early and accurate molecular diagnosis plays a critical role in supporting timely clinical decision-making and patient management.

The J. ENCEPHALITIS- Q Real-Time PCR Kit helps laboratories to:

Targets Detected

Qualitative Targets
  • · Japanese encephalitis virus (JEV) RNA
Internal Control
  • Human RNaseP
    Ensures successful RNA extraction, assay integrity, sample adequacy, and reliable amplification for every clinical sample.

Applications

The kit is ideal for clinical molecular laboratories performing:

j.encephalia-Q Real-Time PCR Kit
  • Japanese encephalitis molecular detection
  • Early molecular diagnosis of Japanese encephalitis infection
  • Confirmation of clinically suspected Japanese encephalitis cases
  • Routine molecular testing in endemic and outbreak settings
  • Integration into infectious disease molecular diagnostic workflows

Key Features & Benefits

Automated Sample-to-Result Workflow:

Integrated with the OnePCR Ultra-Fast RT PCR System for automated nucleic acid extraction, amplification, and result interpretation, significantly reducing manual processing and hands-on time.

Sensitive and Specific Detection:

Utilizes target-specific primers and fluorescent probe-based chemistry for highly sensitive and specific qualitative detection of Japanese encephalitis virus RNA, enabling reliable detection even in samples with low viral loads.

Single-Tube Multiplex Assay

Employs a multiplex Real-Time RT-PCR assay for simultaneous detection of Japanese encephalitis virus RNA and the Human RNaseP internal control within a single reaction tube.

Internal Control for Quality Assurance

Built-in Human RNaseP Internal Control verifies successful nucleic acid extraction, sample quality, and assay performance, ensuring confidence in every reported result.

Sample Types & Reporting Output

Sample Types

Validated for use with:

  • Cerebrospinal Fluid (CSF)
  • Serum
Reporting Output

The test provides clear and actionable laboratory results, including:

  • Positive / Negative interpretation
  • Ct values
  • Amplification curves
  • Human RNaseP Internal Control status
  • Result export options (USB or Bluetooth via OnePCR System)

Compatible System

OnePCR Ultra-Fast RT PCR System

Designed for automated nucleic acid extraction, Real-Time RT-PCR amplification, and integrated result reporting through a rapid sample-to-result workflow.

ONEPCR Automated Testing Workflow
  • Collect CSF or serum sample.
  • Load 200 μL of the sample into Cartridge-1.
  • Reconstitute Cartridge-2 by adding 10 μL Reconstitution Buffer.
  • Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast RT PCR System.
  • Fully automated RNA extraction and Real-Time RT-PCR amplification.
  • Results displayed with Ct values, amplification curves, and Positive/Negative interpretation.

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
J. ENCEPHALITIS- Q Real-Time PCR Kit G2M802021R 24 Tests

Why Choose the J. ENCEPHALITIS- Q Real-Time PCR Kit?

If your laboratory requires a reliable molecular assay for rapid detection of Japanese encephalitis virus RNA, the J. ENCEPHALITIS- Q Real-Time PCR Kit delivers:

  • automated RNA extraction and Real-Time RT-PCR workflow
  • built-in Human RNaseP Internal Control for assay validation
  • reliable and accurate diagnostic performance
  • minimal hands-on time with an integrated sample-to-result workflow
  • seamless integration into infectious disease molecular testing workflows using the OnePCR Ultra-Fast RT PCR System

Product Enquiry Form

Other Products

Rapi-Q Series
Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

Rapi-X96
Rapi-X96

High Throughput fully Automated Nucleic Acid Extraction System.

TRapi-X96
Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

© 2025 Genes2me. All rights reserved.