COVIDtect Multiplex RT-PCR Kit

Qualitative Detection of SARS-CoV-2 RNA

The COVIDtect Multiplex RT-PCR Kit is an in vitro diagnostic (IVD) molecular diagnostic assay designed for the qualitative detection of SARS-CoV-2 RNA, including the identification of the Omicron variant (through S gene dropout) and other circulating SARS-CoV-2 variants with RdRp, S, and N gene targets from human nasopharyngeal and oropharyngeal swab specimens.

Designed for use with the OnePCR Ultra-Fast POC RT-PCR System, the assay provides rapid, sensitive, and highly specific molecular detection of SARS-CoV-2, enabling early diagnosis, timely patient management, infection control, and public health surveillance.

Product Overview

The COVIDtect Multiplex RT-PCR Kit is an advanced multiplex Real-Time Reverse Transcription PCR (RT-PCR) assay developed for the qualitative detection of SARS-CoV-2 RNA. The assay integrates automated RNA extraction, reverse transcription, amplification, and result interpretation through the OnePCR Ultra-Fast POC RT-PCR System, delivering a true sample-to-result workflow with minimal user intervention.

The multiplex assay simultaneously detects multiple viral targets to improve diagnostic accuracy while minimizing the possibility of false-negative results caused by viral mutations.

The kit detects:

  • RdRp Gene
  • N Gene
  • S Gene
  • Human RNase P Internal Control

The inclusion of multiple viral gene targets provides robust detection across circulating SARS-CoV-2 variants while allowing identification of the Omicron variant through S gene target dropout, supporting effective clinical diagnosis and epidemiological monitoring.

COVIDtect Multiplex RT-PCR Kit

Why SARS-CoV-2 Testing Matters

Coronavirus Disease 2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), remains one of the most significant global infectious diseases. Transmission occurs primarily through respiratory droplets and aerosols, with infected individuals ranging from completely asymptomatic to critically ill.

Clinical manifestations commonly include fever, cough, sore throat, fatigue, muscle pain, shortness of breath, and loss of taste or smell. In severe cases, infection may progress to pneumonia, Acute Respiratory Distress Syndrome (ARDS), sepsis, multi-organ dysfunction, and death, particularly among elderly individuals and patients with underlying medical conditions. Because clinical symptoms overlap with many respiratory viral infections, molecular diagnosis using Real-Time RT-PCR remains the gold standard for confirming SARS-CoV-2 infection.

The COVIDtect Multiplex RT-PCR Kit enables laboratories to:

Targets Detected

Viral Targets
  • RdRp Gene
  • N Gene
  • S Gene
Internal Control
  • Human RNase P (HEX Channel)
    The Internal Control verifies proper sample collection, RNA extraction, amplification efficiency, and overall assay performance, ensuring reliable interpretation of every test result.

Applications

The kit is ideal for molecular laboratories performing:

Nipah-Q Real-Time PCR Kit
  • COVID-19 molecular diagnosis
  • Early detection of SARS-CoV-2 infection
  • Omicron variant screening
  • Respiratory virus surveillance
  • Outbreak investigation
  • Point-of-care molecular testing
  • Hospital and public health laboratory diagnostics

Key Features & Benefits

Fully Automated Sample-to-Result Workflow

Integrated with the OnePCR Ultra-Fast POC RT-PCR System, enabling automated RNA extraction, reverse transcription, amplification, fluorescence detection, and result interpretation without complex laboratory procedures.

Multiplex Detection for Enhanced Diagnostic Accuracy

Simultaneously detects three independent SARS-CoV-2 genetic targets (RdRp, N, and S genes), improving assay robustness and reducing the likelihood of false-negative results associated with emerging viral mutations.

Omicron Variant Identification

The assay identifies suspected Omicron variant infections through S gene target dropout, while confirming infection using the RdRp and N gene targets, supporting variant surveillance without additional testing.

High Sensitivity and Specificity

Utilizes optimized primers and fluorescent hydrolysis probes for accurate detection of SARS-CoV-2 RNA, even in specimens with low viral loads.

Integrated Internal Quality Control

Human RNase P serves as an internal control to verify specimen adequacy, nucleic acid extraction efficiency, amplification performance, and overall assay validity.

Rapid Turnaround Time

The automated workflow significantly reduces hands-on time while delivering rapid and reproducible molecular results, making the assay suitable for decentralized and point-of-care molecular diagnostics.

Sample Types & Reporting Output

Sample Types

Validated for use with:

  • Nasopharyngeal Swab
  • Oropharyngeal Swab
Reporting Output

The assay generates comprehensive laboratory reports including:

  • Positive / Negative interpretation
  • Ct values for each detected target
  • Amplification curves
  • Internal Control status
  • USB and Bluetooth data export through the OnePCR System

Compatible System

OnePCR Ultra-Fast POC RT-PCR System

The assay is specifically optimized for the OnePCR Ultra-Fast POC RT-PCR System, providing a fully automated workflow from nucleic acid extraction through Real-Time RT-PCR amplification to result interpretation in a single integrated platform.

ONEPCR Automated Testing Workflow
  • Collect nasopharyngeal or oropharyngeal swab specimens using sterile collection devices.
  • Add 200 µL of the specimen into Cartridge-1.
  • Reconstitute Cartridge-2 using 10 µL Reconstitution Buffer.
  • Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast POC RT-PCR System.
  • The system automatically performs RNA extraction, reverse transcription, multiplex RT-PCR amplification, fluorescence detection, and data analysis.
  • Results are displayed as Positive or Negative, together with Ct values, amplification curves, and internal control status.

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
COVIDtect Multiplex Real-Time PCR Kit G2M030521 24 Tests

Why Choose the COVIDtect Multiplex RT-PCR Kit?

For laboratories seeking a rapid, accurate, and automated molecular solution for COVID-19 diagnosis, the COVIDtect Multiplex RT-PCR Kit offers:

  • Fully automated sample-to-result workflow on the OnePCR platform
  • Multiplex detection of RdRp, N, and S genes for enhanced diagnostic confidence
  • Omicron variant identification through S gene dropout analysis
  • High analytical sensitivity and specificity
  • Integrated Human RNase P Internal Control for assay validation
  • Minimal hands-on time with rapid turnaround
  • Reliable performance for routine clinical testing, outbreak response, and public health surveillance
  • Seamless integration with the OnePCR Ultra-Fast POC RT-PCR System

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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