M.Pox(VZV)-Q Real-Time PCR Kit

Qualitative Detection of Varicella-Zoster Virus & Monkeypox Virus DNA

M.Pox(VZV)-Q Real-Time PCR Kit is an In Vitro Diagnostic (IVD) Molecular Diagnostic test based on the Real-Time PCR (Polymerase Chain Reaction) technique for the Qualitative detection of Varicella Zoster Virus specific DNA in clinical samples. The test is also capable of identifying and detecting Monkeypox virus from Varicella-Zoster Virus (VZV).

Product Overview

M. Pox (VZV) -Q Real-Time PCR Kit is the qualitative detection kit which adopts advanced real-time PCR technology for the detection of Varicella-Zoster Virus (VZV) and Monkeypox virus DNA from human clinical samples such as lesion surface and lesion crusts. The kit is recommended for use with the OnePCR Ultra-Fast POC Rapid RT-PCR System for the automation of the DNA extraction and Real-Time PCR processes.

This kit enables the simultaneous detection of:

  • Monkeypox virus Clade I specific DNA
  • Monkeypox virus Clade II specific DNA
  • Varicella-Zoster virus (VZV) specific DNA
  • Internal Control (RNaseP gene) for assay validation and quality control of the testing process.

The kit is a single-tube assay which employs specifically designed primers and hybridization probes for the simultaneous target-specific detection of Monkeypox virus Clade I, Monkeypox virus Clade II and Varicella-Zoster Virus specific genes.

VZV

Why VZV & Monkeypox Testing Matters

Mpox (previously referred to as Monkeypox) is caused by the Monkeypox virus. The virus is an enveloped double-stranded DNA virus of the Orthopoxvirus genus in the family Poxviridae. Monkeypox is a zoonotic viral disease that is transmitted from animals to humans and can also be transmitted from human to human, causing a rash on the skin.

Herpes Zoster is a disease caused by the Varicella Zoster Virus which is very contagious; therefore, the RT-PCR testing at an early stage is of great significance. Using the M.Pox (VZV) -Q Real-TimePCR Kit helps laboratories:

Targets Detected

Qualitative Targets
  • Monkeypox virus Clade I DNA
  • Monkeypox virus Clade II DNA
  • Varicella-Zoster Virus (VZV) DNA
Internal Control
  • Internal Control (IC)
    RNaseP gene, for successful DNA extraction, integrity of the sample and assay, and reliable amplification for each sample.

Applications

The M. Pox (VZV) -Q Real-Time PCR Kit is suitable for use in clinical molecular labs for:

M. hominis Real-Time PCR Kit
  • Molecular detection and differentiation of VZV and Monkeypox virus infections
  • Early molecular diagnosis for Herpes Zoster
  • Early molecular diagnosis and differential diagnosis of Mpox (Monkeypox) infections and determination of virus clades
  • Infectious disease and outbreak response molecular diagnostic testing

Key Features & Benefits

Automated Sample-to-Result Workflow with Rapid Results

Integrated with the OnePCR Ultra-Fast POC Rapid RT-PCR System for automated DNA extraction, amplification, and result interpretation with minimal hands-on time.

Sensitive and Specific Detection

Single Reaction for Simultaneous Differential Detection: Simultaneous detection and differentiation of VZV, Monkeypox virus Clade I and Clade II in one reaction.

Sensitive and Specific Detection

Employ multi-dye fluorescent probe-based chemistry combined with VZV, Monkeypox virus Clade I&II target-specific primers for highly sensitive and specific target detection.

Built-in Internal Control for Quality Assurance

Internal Control Assay for Reliable Performance: Assay includes RNaseP Internal Control to verify successful DNA extraction, sample adequacy, and accurate assay performance.

Analytical and Clinical Performance Characteristics

Assay shows high analytical sensitivity, analytical specificity, repeatability, and reproducibility as well as high clinical sensitivity and specificity.

Sample Types & Reporting Output

Sample Types

Validated for use with:

  • Human clinical samples such as lesion swabs and tissue are recommended for use with this kit.
Reporting Output

Interpretation of laboratory results for this test includes:

  • Positive / Negative indication for each target (MPXV Clade I, MPXV Clade II, VZV)
  • Ct values
  • Amplification curves
  • Internal Control (RNAseP) result
  • Result export option (USB or Bluetooth)

Compatible System

OnePCR: OnePCR Ultra-Fast POC Rapid RT-PCR System (Genes 2Me Private Limited)

The system was designed based on an automated workflow which allows sample DNA extraction and RT-PCR amplification.

ONEPCR Automated Testing Workflow
  • Collect the lesion swab / tissue sample following the sample preparation guidelines.
  • Load 200 µL sample into well 'A' (sample well) of Cartridge-1.
  • Reconstitute Cartridge-2 by adding 10 µL Reconstitution Buffer
  • Place Cartridge-1 and Cartridge-2 in the OnePCR Ultra-Fast POC Rapid RT-PCR System.
  • Run the machine to perform automated DNA extraction and Real-Time PCR testing.
  • Interpret results with Ct values and amplification curves indicating Positive or Negative results for Monkeypox virus Clade I, Monkeypox virus Clade II and VZV infection.

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
M.Pox(VZV)-Q Real-Time PCR Kit G610011 G610011-3 24 Tests

Why Choose the VZV-Q Real-Time PCR Kit?

If you are looking for an IVD molecular diagnostic test for rapid detection of VZV and Monkeypox virus, the M. Pox (VZV) -Q Real-Time PCR Kit offers:

  • automated DNA extraction and Real-Time PCR workflow
  • simultaneous detection and differentiation of VZV, Monkeypox Clade I and Monkeypox Clade II
  • built-in RNaseP Internal Control for assay validation
  • minimal hands-on time and fully automated Sample-to-Result workflow
  • suitable for use in the workflow of infectious disease molecular diagnostic testing

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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