Vaginalis (TGC III)-Q Real-Time PCR Kit | Reproductive Health IVD RT-PCR Testing

Product Overview

The Vaginalis (TGC III)-Q Real-Time PCR Kit is an in vitro diagnostic assay based on real-time PCR technology for detecting vaginitis in vaginal swab samples. The kit works seamlessly with the OnePCR Ultra-Fast POC Rapid RT-PCR System, which automates the entire process of DNA extraction and real-time PCR analysis.

The kit detects:

Trichomonas vaginalis (TV) specific DNA
Gardnerella vaginalis (G. vaginalis) specific DNA
Candida spp. specific DNA
Internal Control (IC) for validating the assay and ensuring quality

The kit includes specific primers and fluorescent probes that target T. vaginalis, G. vaginalis, and Candida spp. By using probes linked to different dyes, the system allows for the simultaneous qualitative detection of T. vaginalis, G. vaginalis, Candida spp., and the internal control.

Why Vaginitis Testing Matters

Trichomonas vaginalis is an anaerobic protozoan parasite responsible for Trichomoniasis. It is the most common pathogenic infection, usually transmitted via direct skin contact with an infected person, often through vaginal intercourse. Gardnerella vaginalis is another anaerobic bacterium found in vaginal flora. It can overgrow, becoming the dominant species, which leads to bacterial vaginosis. This is the most common cause of vaginal discharge and increases the risk of acquiring HIV and other STIs.

Candida spp. are common fungal pathogens that cause Candidal vulvovaginitis when they penetrate the mucosal lining of the vagina. This invasion triggers inflammation, which results in thick discharge, vaginal itching, and burning. The symptoms of these infections are quite similar, making clinical diagnosis challenging and complicating risk assessment during pregnancy. The Vaginalis (TGC III)-Q Real-Time PCR Kit assists laboratories by:

rapidly detecting T. vaginalis, G. vaginalis, and Candida spp. specific DNA
differentiating among the common causes of vaginitis
supporting early clinical diagnosis
facilitating timely patient management
strengthening laboratory-based infectious disease diagnosis

Targets Detected

Qualitative Targets

Trichomonas vaginalis (TV) DNA
Gardnerella vaginalis DNA
Candida spp. DNA

Internal Control

Internal Control (IC)
The Internal Control ensures successful DNA extraction, assay integrity, sample adequacy, and reliable amplification for every sample.

Applications

The kit is ideal for clinical molecular laboratories performing:

Molecular detection of organisms causing vaginitis
Early molecular diagnosis of Trichomoniasis, bacterial vaginosis, and Candidal vulvovaginitis
Routine molecular testing of vaginal swabs
Differentiating among clinically similar vaginitis presentations
Screening for risk factors during pregnancy
Integration into molecular diagnostic workflows for infectious diseases

Key Features & Benefits

Automated Sample-to-Result Workflow

Integrated with the OnePCR Ultra-Fast POC Rapid RT-PCR System for automated DNA extraction, amplification, and result interpretation while minimizing hands-on time.

Simultaneous Multi-Target Detection

Detects three major causes of vaginitis—T. vaginalis, G. vaginalis, and Candida spp.—in a single reaction, aiding in the differential diagnosis of similar presentations.

Sensitive and Specific Detection

Uses target-specific primers and multi-dye fluorescent probe-based chemistry for highly sensitive and specific detection.

Built-in Internal Control for Quality Assurance

The built-in Internal Control verifies successful DNA extraction, sample quality, and assay performance, instilling confidence in each reported result.

Optimized for Routine Clinical Testing

Designed for rapid and dependable molecular diagnosis in clinical labs conducting routine infectious disease testing.

Sample Types & Reporting Output

Sample Types

Validated for use with:

vaginal swabs.

Reporting Output

The test provides clear and actionable laboratory results, including:

Positive / Negative interpretation for each target (T. vaginalis, G. vaginalis, Candida spp.)
Ct values
Amplification curves
Internal Control status
Result export options (USB or Bluetooth)

Compatible System

OnePCR Ultra-Fast POC Rapid RT-PCR System (Genes 2Me Private Limited)

Designed for a fully automated workflow that combines DNA extraction and real-time PCR amplification.

Collect the vaginal swab / clinical sample.
Load 200 µL of the sample into well 'A' (marked 'Sample') of Cartridge-1.
Reconstitute Cartridge-2 by adding and mixing 10 µL Reconstitution Buffer.
Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast POC Rapid RT-PCR System.
The system will fully automate DNA extraction and real-time PCR amplification run.
Results will display with Ct values, amplification curves, and Positive/Negative interpretation for each target.

Storage & Stability

Store at 2°C – 8°C
Ship at 10°C–30°C
Shelf life: 12 months from the date of manufacture
Stable under recommended storage and transportation conditions
Protect from direct sunlight

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Vaginalis (TGC III)-Q Real- Time PCR Kit		G610012-3	24 Tests

Why Choose the T. Vaginalis-Q Real-Time PCR Kit?

If your laboratory needs a reliable molecular assay for rapid detection of the main organisms causing vaginitis, the Vaginalis (TGC III)-Q Real-Time PCR Kit provides:

automated DNA extraction and real-time PCR workflow
simultaneous detection of T. vaginalis, G. vaginalis, and Candida spp.
reliable and precise diagnostic performance
minimal hands-on time with an integrated sample-to-result workflow
seamless integration into infectious disease molecular testing workflows

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Qualitative Detection of Trichomonas vaginalis, Gardnerella vaginalis & Candida spp. DNA

Product Overview

Why Vaginitis Testing Matters