Syphilis-Q Real-Time PCR Kit

Qualitative Detection of Treponema pallidum DNA

The Syphilis-Q Real-Time PCR Kit is an in vitro diagnostic test that uses real-time PCRtechnology. It is designed to qualitatively detect Syphilis DNA in human clinicalsamples. This assay allows for quick and accurate detection of Treponema pallidum(TP), the bacterium that causes Syphilis. It follows a standardized real-time PCRprocess that supports timely diagnosis and effective management of Syphilis.

Product Overview

The Syphilis-Q Real-Time PCR Kit is an in vitro diagnostic assay that uses real-time PCRtechnology to detect conserved regions within the DNA of Treponema pallidum (TP). Thekit integrates seamlessly with the OnePCR Ultra-Fast POC Rapid RT-PCR System, whichautomates the entire DNA extraction and PCR analysis process.

The kit detects:

  • Treponema pallidum (TP) DNA
  • Human RNaseP Internal Control (IC) for validation and quality assurance

The assay uses a single-tube multiplex Real-Time PCR format to detect Syphilis DNA.Detection is achieved through fluorescent oligonucleotide probes labeled with FAM,ensuring high sensitivity and specificity.

Syphilis-Q

Why Syphilis Testing Matters

Syphilis is caused by the bacterium T. pallidum and spreads primarily through contactwith an infected person's sore during sexual activity. The bacteria enter the bodythrough small cuts or abrasions in the skin or mucous membranes. It can also bepassed to a fetus during later stages of pregnancy, leading to congenital Syphilis.

Regular testing and prompt treatment are vital for managing Syphilis and preventingcomplications. While serology tests may lack sensitivity at low levels of the pathogen,real-time PCR is very sensitive and specific. It amplifies bacterial DNA for accurateidentification, even at low pathogen densities. The Syphilis-Q Real-Time PCR Kit assists laboratories in:

Targets Detected

Qualitative Targets
  • Treponema pallidum (TP) DNA
  • Internal Control (IC)
Internal Control
  • Internal Control (IC)
    Human RNaseP ensures successful DNA extraction, assay integrity, sample adequacy,and reliable amplification for every sample.

Applications

The kit is ideal for clinical molecular laboratories performing:

Syphilis-Q
  • Syphilis molecular detection
  • Early molecular diagnosis of Syphilis infection
  • Routine Syphilis molecular testing
  • Confirmation of suspected Syphilis cases
  • Integration into molecular diagnostic workflows for infectious diseases

Key Features & Benefits

Fully Automated Sample-to-Result Workflow

The kit is integrated with the OnePCR Ultra-Fast POC RT-PCR System. This allowsautomated RNA extraction, amplification, fluorescence detection, and resultinterpretation without complex lab procedures.

Sensitive and Specific Detection

It uses target-specific primer-probe sets and fluorescent oligonucleotide probe-basedchemistry (FAM) for highly sensitive and specific detection of T. pallidum DNA.

Single-Tube Multiplex Assay

It employs a single- tube multiplex Real-Time PCR assay to detect the Syphilis targetand Human RNaseP internal control simultaneously.

Broad Sample Compatibility

This kit targets extracted DNA from human whole blood, cerebrospinal fluid, amnioticfluid, and genitourinary ulcers.

Optimized for Routine Clinical Testing

It is designed for rapid and reliable molecular diagnosis in labs that conduct routineinfectious disease testing.

Sample Types & Reporting Output

Sample Types

Validated for use with DNA extracted from:

  • Human whole blood (EDTA)
  • Cerebrospinal fluid
  • Amniotic fluid
  • Genitourinary ulcers
Reporting Output

The test provides clear and actionable laboratory results, including:

  • Positive / Negative interpretation
  • Ct values
  • Amplification curves
  • Human RNaseP Internal Control status
  • Result export options (USB or Bluetooth)

Compatible System

OnePCR: OnePCR Ultra-Fast POC Rapid RT-PCR System

This system is designed for an automated workflow that combines sample DNAextraction and Real-Time PCR amplification.

ONEPCR Automated Testing Workflow
  • Collect 3–5 ml of venous blood into a sterile EDTA tube.
  • Load 200 μL of the sample into well 'A' (marked 'Sample') of Cartridge-1.
  • Reconstitute Cartridge-2 by adding 10 μL Reconstitution Buffer and attach the tubeadaptor.
  • Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast POC Rapid RT-PCRSystem.
  • Automatic DNA extraction and Real-Time PCR amplification will take place.
  • Results will display with Ct values, amplification curves, and Positive/Negativeinterpretation.

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
Syphilis-Q Real Time PCR Kit G610048 G610048-3 24 Tests

Why Choose the Syphilis-Q Real-Time PCR Kit?

If your lab needs a reliable molecular test for quick detection of Treponema pallidumDNA, the Syphilis-Q Real-Time PCR Kit offers:

  • Automated DNA extraction and PCR workflow with the OnePCR system
  • Highly sensitive and specific probe-based detection
  • Built-in Human RNaseP Internal Control for validation
  • Reliable and accurate diagnostic performance
  • Suitability for routine testing of Syphilis
  • Easy integration into infectious disease molecular testing workflows
  • smooth integration into infectious disease molecular testing workflows

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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