HSV-Q Real-Time PCR Test Kit

Qualitative Detection of Herpes Simplex Virus 1 & 2 DNA

The HSV-Q Real-Time PCR Test Kit is an in vitro diagnostic (IVD) molecular test designedto detect Herpes Simplex Virus 1 (HSV-1) and Herpes Simplex Virus 2 (HSV-2) DNA inclinical samples.

This test allows for quick detection and differentiation of HSV-1 and HSV-2 through afully automated Real-Time PCR process, aiding timely diagnosis and proper clinical management.

Product Overview

The HSV-Q Real-Time PCR Test Kit is an in vitro diagnostic (IVD) assay based on Real-Time PCR technology. It is designed to work seamlessly with the OnePCR Ultra-FastPOC Rapid RT-PCR System, which automates DNA extraction and real-time PCRanalysis in a single closed cartridge run.

The kit includes specific primers and fluorescent probes that target the HSV-1glycoprotein D gene and the HSV-2 US5 gene. The kit detects:

  • Herpes Simplex Virus 1 (HSV-1) DNA
  • Herpes Simplex Virus 2 (HSV-2) DNA
  • Internal Control for assay validation and quality assurance

The assay uses oligonucleotide probes labeled with two different fluorescent dyes. Thisallows for parallel detection of HSV-1 and HSV-2 and the internal control in a single automated run.

HSV-Q Real-Time PCR Test Kit

Why HSV Testing Matters

Herpes is a common viral infection caused by Herpes Simplex Virus 1 (HSV-1) andHerpes Simplex Virus 2 (HSV-2). These viruses belong to the Orthoherpesviridae family. HSV-1 and HSV-2 share over 80% nucleotide identity in their protein coding regions. HSV-1 mainly spreads through oral contact and typically causes oral herpes or coldsores, though it can also lead to genital herpes. In contrast, HSV-2 primarily spreadsthrough sexual contact and causes genital herpes.

Molecular testing is vital for accurate diagnosis and differentiation between HSV-1 andHSV-2.The HSV-Q Real-Time PCR Test Kit helps laboratories to:

Targets Detected

Qualitative Targets
  • Herpes Simplex Virus 1 (HSV-1)
  • Herpes Simplex Virus 2 (HSV-2)
Internal Control
  • Internal Control (IC)
    This ensures successful nucleic acid extraction, assay integrity, and reliableamplification for every sample.

Applications

The kit is ideal for clinical molecular laboratories performing:

M. hominis Real-Time PCR Kit
  • Herpes Simplex Virus molecular detection
  • Differentiation of HSV-1 and HSV-2 infections
  • Diagnosis of oral and genital herpes infections
  • Confirmation of suspected HSV-associated infections
  • Integration into infectious disease molecular diagnostic workflow

Key Features & Benefits

Automated Sample-to-Result Workflow

The kit is integrated with the OnePCR Ultra-Fast POC Rapid RT-PCR System for automated DNA/RNA extraction, amplification, andresult interpretation with minimal hands-on time.

Sensitive and Specific Detection

It uses target-specific primers and fluorescentprobe-based chemistry for accurate detection of HSV-1 and HSV-2.

Dual-Type Differentiation

Dual-Type Differentiation: The assay simultaneously distinguishes HSV-1 from HSV-2in a single closed-cartridge run.

Built-in Internal Control for Quality Assurance

Internal Control for Quality Assurance: The built-in Internal Control (Cy5) confirmssuccessful extraction, sample quality, and assay performance.

Closed-System, Contamination-Resistant Design

Closed-System, Contamination-Resistant Design: The cartridge-based workflowreduces hands-on steps and lowers the risk of cross-contamination

Optimized for Routine Clinical Testing

This kit is designed for rapid, reliablemolecular diagnosis in clinical laboratories that perform routine infectious diseasetesting.

Sample Types & Reporting Output

Sample Types

Validated for use with:

  • Whole blood
  • Plasma
  • Serum
Reporting Output

The test provides clear and useful laboratory results, including:

  • ositive / Negative interpretation for each targe
  • Ct values
  • Amplification curves
  • Internal Control status
  • Result export options (USB or Bluetooth via OnePCR System)

Compatible System

OnePCR Ultra-Fast POC Rapid RT-PCR System

This system is designed for a fully automated workflow that includes DNA/RNAextraction and real-time PCR amplification in a single closed cartridge run.

ONEPCR Automated Testing Workflow
  • Collect a blood, plasma, or serum sample following the sample collection guidelines.
  • Load 200 μL of the sample into Cartridge-1 (well 'A', marked 'Sample').
  • Reconstitute Cartridge-2 by adding 10 μL of Reconstitution Buffer and attach the tubeadapter.
  • Load Cartridge-1 and Cartridge-2 into the OnePCR Ultra-Fast POC RT-PCR System.
  • The system will perform fully automated DNA/RNA extraction and Real-Time PCRamplification.
  • Results are displayed with Ct values, amplification curves, and Positive/Negativeinterpretation for HSV-1 and HSV-2.

Storage & Stability

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
HSV-Q Real-Time PCR Test Kit 610009-3 24 Tests

Why Choose the HSV-Q Real-Time PCR Test Kit?

If your laboratory needs a reliable molecular test for the quick detection anddifferentiation of Herpes Simplex Virus 1 and 2, the HSV-Q Real-Time PCR Test Kit offers:

  • Automated DNA/RNA extraction and Real-Time PCR workflow with the OnePCRsystem
  • Highly sensitive and specific probe-based detection
  • Built-in Internal Control for assay validation
  • Reliable and accurate diagnostic performance
  • Minimal hands-on time with an integrated, closed-system sample-to-result workflow
  • Suitability for routine herpes infection molecular diagnostics
  • Seamless integration into infectious disease molecular testing workflows

Product Enquiry Form

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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