SCA-Q Real-Time PCR Kit - Genes2Me™ | Sickle Cell Anemia Detection

Product Overview

The SCA-Q Real-Time PCR Kit is a diagnostic test designed to screen the human beta-globin GAG-GTG [Glu-Val] mutation linked to sickle cell anemia. The test uses specific primers and probes to accurately amplify and directly detect the mutation. It includes a wild-type gene target as an internal control to verify effective DNA purification and to rule out PCR inhibition.

Why Sickle Cell Mutation Testing Matters

Sickle cell anemia is a hereditary condition involving structural changes in red blood cells, which prevent them from functioning properly.

Molecular mutation detection allows:

Targeted detection of the mutation causing sickle cell anemia
Detection of the human beta-globin GAG-GTG [Glu-Val] mutation
Molecular analysis of the targeted mutation

Targets Detected

Genetic Target

Human beta-globin GAG-GTG [Glu-Val] mutation

Internal Control

Internal Control (IC)
Wild-type gene target for assessment of DNA purification efficiency and PCR inhibition

Applications & Use Scenarios

The kit is intended for use in clinical laboratories for:

Molecular detection of sickle cell anemia mutation
Genetic testing workflows
Molecular assessment of beta-globin mutation
Support for diagnosis of sickle cell anemia

Key Features & Benefits

Targeted detection of the mutation causing sickle cell anemia

Detection of the human beta-globin GAG-GTG [Glu-Val] mutation

Real-time PCR-based molecular detection

Wild type gene internal control

PCR inhibition and DNA extraction quality evaluation

Direct mutation detection

Sample Types & Reporting Output

Sample Types

Validated for use with:

Venous blood collected in EDTA tubes

Reporting Output

The test provides molecular findings based on amplification of the target mutation and internal control, including:

Detection status of the sickle cell anemia mutation
Target amplification results
Wild-type gene / Internal Control status
Assessment of PCR inhibition and DNA purification efficiency
Amplification curves

Compatible System

The SCA-Q Real-Time PCR Kit is compatible with:

Designed for real-time PCR-based detection of the targeted sickle cell anemia mutation.

QuantStudio™ 5 Real-Time PCR System — Thermo Fisher Scientific
CFX96 Real-Time PCR System — Bio-Rad
LightCycler® 480 System — Roche Diagnostics
RapiCycler 96 Real Time PCR System — Genes 2Me Private Limited

Storage & Stability

Shelf life: 12 months from date of manufacture
Storage temperature: -20°C
Shipped at: -30°C to -10°C
Experiment setup: 15°C to 30°C
Keep reagents in coolant at: 2°C to 8°C during testing
Store reagents upright at: -30°C to -10°C
Avoid more than 6 freeze-thaw cycles
Protect from direct sunlight

Commercial Name	Old Cat No.	New Cat No.	Pack Size
SCA-Q Real Time PCR Kit		G2M803221-1	50 Tests

Commercial Name

Old Cat No.

New Cat No.

Pack Size

SCA-Q Real Time PCR Kit

G2M803221-1

50 Tests

Why choose the SCA-Q Real time PCR Kit?

If your lab requires a targeted molecular assay for sickle cell anemia mutation detection, this assay delivers:

Targeted detection of the beta-globin GAG-GTG [Glu-Val] mutation
Real-time PCR-based molecular testing
Wild-type gene internal control for process validation
Assessment of PCR inhibition and DNA purification efficiency
Compatibility with multiple real-time PCR platforms
suitability for routine M. hominis molecular diagnostics
Direct molecular detection of the sickle cell anemia mutation

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Molecular Detection of Sickle Cell Anemia Mutation by Real-Time PCR

Product Overview

Why Sickle Cell Mutation Testing Matters