Yellow Fever (YF) is an acute mosquito-borne viral disease caused by the Yellow Fever virus (YFV), a member of the Flavivirus genus. Transmitted primarily by Aedes aegypti and other mosquito species, Yellow Fever continues to pose a major public health threat across tropical regions of Africa and South America. Characterized by a spectrum of clinical stages—ranging from initial fever, headache, nausea, and jaundice to severe intoxication with multi-organ failure, bleeding, seizures, and coma—the disease has epidemic potential and can be fatal if not diagnosed and managed promptly. Despite being vaccine-preventable, outbreaks still occur, making early and accurate detection critical for both patient survival and outbreak control.
The Y. Fever-Q Real-Time PCR Kit from Genes2Me is a highly sensitive and specific in vitro diagnostic assay designed for the qualitative detection of Yellow Fever virus RNA from human blood, serum, or plasma samples.
Genes2Me is a CE Marked molecular diagnostics company trusted by clinical laboratories across global markets. The Y. Fever-Q Real-Time PCR Kit meets European conformity standards for in vitro diagnostic devices, validated to international benchmarks for accuracy, reliability, and safety.
The Y. Fever-Q Kit is based on real-time reverse transcription PCR (RT-PCR), targeting the polyprotein gene region of the YFV genome for precise detection. FAM-labeled probes detect YFV RNA in the FAM channel, while an Internal Control based on the RNaseP housekeeping gene is monitored in the Cy5 channel to validate the complete process from extraction through amplification. his design eliminates false negatives arising from poor sample quality or PCR inhibition and enhances overall result reliability. Positive and negative controls further safeguard assay performance, guaranteeing confidence in every test. Ready-to-use reagents and a straightforward protocol minimise hands-on time and reduce the potential for procedural errors.
Yellow Fever infection frequently presents with non-specific febrile illness, making clinical diagnosis unreliable, particularly in early stages. Serological testing is further complicated by cross-reactivity with related flaviviruses such as Dengue, Zika, and West Nile virus. Molecular detection of YFV RNA provides the earliest confirmation of active infection, ahead of antibody seroconversion, and is the recommended approach during the acute viremic phase. Accurate and timely diagnosis is essential not only for patient management but also for outbreak containment and the implementation of targeted public health responses.
Detects YFV RNA with a Ct cut-off of 35 or below, ensuring accurate diagnosis even at low viral loads and during early or subclinical infection.
Built-in RNaseP housekeeping gene control monitored in the Cy5 channel validates sample quality and assay integrity across the complete workflow from extraction through amplification.
No false positives observed with closely related arboviruses including Dengue, Zika, or West Nile virus, confirming high assay specificity in clinically relevant differential diagnosis scenarios.
Validated across leading real-time PCR systems including QuantStudio 5, Bio-Rad CFX96, Roche LightCycler 480, and Genes2Me's RapiCycler 96, supporting seamless integration into existing laboratory workflows.
Pre-formulated reagents with a 12-month shelf life under recommended storage conditions minimise setup time, reduce procedural errors, and simplify inventory management with stable cold-chain logistics.
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| Y. Fever-Q Real-Time PCR Kit | G2M801821 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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