Para-Q Multiplex Real-Time PCR Kit

Product Overview


Vector-borne diseases such as Dengue, Malaria, and Chikungunya continue to impose a massive health burden across tropical and subtropical regions. Transmitted by mosquitoes, these infections frequently present with overlapping symptoms including high fever, chills, joint pain, and rashes, making accurate diagnosis extremely challenging through conventional methods. Misdiagnosis delays treatment and increases the risk of complications, severe illness, and outbreaks. With early and reliable detection being the cornerstone of effective management, there is an urgent need for advanced diagnostic solutions capable of simultaneously identifying these pathogens with speed and precision.

The Para-Q Multiplex Real-Time PCR Kit from Genes2Me is an innovative in vitro diagnostic assay designed for the qualitative detection of nucleic acids specific to Dengue virus, Malaria parasite, and Chikungunya virus in a single test. By combining advanced multiplex technology with robust quality controls, the kit enables timely diagnosis, effective patient management, and better outbreak preparedness.

Genes2Me is a CE Marked molecular diagnostics company trusted by clinical laboratories across global markets. The Para-Q Multiplex Real-Time PCR Kit meets European conformity standards for in vitro diagnostic devices, validated to international benchmarks for accuracy, reliability, and safety.

Targets:
Dengue Virus, Malaria Parasite, Chikungunya Virus
Technology:
Multiplex One-Step Real-Time PCR
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
EDTA Blood
Certification:
CE-IVD
Para-Q Multiplex

How the Assay Works


The Para-Q kit uses a multiplex real-time PCR format with multiple primer-probe sets, enabling simultaneous detection and differentiation of three pathogens in a single reaction. Each target is assigned a dedicated fluorescence channel:

Target
Gene Target
Detection Channel
Dengue Virus
Polyprotein gene
FAM
Malaria Parasite
18S SSU rRNA gene
HEX
Chikungunya Virus
Polyprotein gene
Texas Red
Internal Control
Workflow validation
Cy5

An Internal Control in the Cy5 channel validates every step from extraction to amplification, ensuring result accuracy and minimising false negatives. A positive control template is included to confirm assay performance, and a no-template control ensures reagent integrity and eliminates contamination risks. Ready-to-use reagents and a straightforward protocol minimise hands-on time and reduce the potential for procedural errors.

Why Molecular Testing Matters for Dengue, Malaria, and Chikungunya


Dengue, Malaria, and Chikungunya share overlapping clinical presentations that make differential diagnosis difficult without laboratory confirmation. Serological testing may lack sensitivity in early infection, and single-pathogen assays require multiple tests and additional time. Multiplex molecular detection of pathogen-specific nucleic acids provides the earliest confirmation of active infection, ahead of antibody seroconversion, and is critical for initiating appropriate treatment promptly. The four-channel detection design with built-in internal control ensures that every negative result reflects a true absence of target pathogens rather than a failed reaction.

Sample Type & Reporting Output


Sample Type:
Blood samples collected in EDTA
Reporting Output:
Qualitative detection of Dengue, Malaria, and Chikungunya nucleic acids (Detected / Not Detected)
Targets Detected:
Dengue Virus RNA, Malaria Parasite DNA/RNA, Chikungunya Virus RNA

Key Features & Benefits


Multiplex one-step real-time PCR

Simultaneously detects and differentiates Dengue virus, Malaria parasite, and Chikungunya virus in a single reaction using dedicated fluorescence channels, saving time and resources compared to running individual assays.

Positive control and NTC included

The positive control validates assay performance in every run, while the no-template control confirms reagent integrity and eliminates contamination risks.

Sensitive detection with Ct cut-off of 35 or below

Reliable identification of low pathogen loads, enabling confident diagnosis during early or subclinical infection.

Internal control across the full workflow

The Cy5-channel internal control validates every step from nucleic acid extraction to amplification, ensuring result reliability and minimising false negatives.

Rapid turnaround

Results available significantly faster than conventional serological or microscopy-based methods, enabling clinicians to initiate timely and targeted patient management.

Compatible with multiple leading PCR platforms

Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480, supporting seamless integration into existing laboratory workflows.

Applications


  • Clinical & Hospital Laboratories: Enables rapid, simultaneous detection of Dengue, Malaria, and Chikungunya from a single EDTA blood sample, supporting accurate differential diagnosis and timely clinical decisions for patients presenting with febrile illness in endemic areas.
  • Paediatric & Neonatal Care: Supports early identification of vector-borne infections in younger patients, where overlapping symptoms and rapid disease progression make prompt molecular confirmation especially critical.
  • Outbreak Surveillance: Rapid multiplex molecular detection supports real-time monitoring of co-circulating pathogens in human populations, enabling targeted public health responses during seasonal outbreak periods.
  • Immunocompromised Patients: Essential for transplant recipients, oncology patients, and others with impaired immunity, where Dengue, Malaria, or Chikungunya infection can result in severe and rapidly progressing disease.
  • Travel & Occupational Health: Reliable multiplex screening for travel medicine clinics and occupational health programmes serving individuals returning from or working in tropical and subtropical endemic regions.
  • Endemic Region Health Systems: Provides high-throughput, cost-effective diagnostic capability for laboratories in regions where co-infection with multiple vector-borne pathogens is a common clinical challenge.

Analytical Performance


  • High analytical sensitivity and specificity for simultaneous detection of Dengue virus, Malaria parasite, and Chikungunya virus
  • Ct cut-off of 35 or below for positive detection, reliable even in low pathogen load samples
  • Endogenous internal control (Cy5) validates the complete workflow from extraction through amplification in every run
  • Positive control and NTC included for full assay integrity and contamination-free validation
  • 12-month shelf life with stable performance under recommended storage conditions

Ordering Information


Commercial Name Old Cat No. New Cat No. Pack Size
Para-Q Multiplex Real-Time PCR Kit G2M706121 50 Tests

Product Enquiry Form


Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.


© 2025 Genes2me. All rights reserved.