Toxoplasmosis is a globally prevalent parasitic infection caused by Toxoplasma gondii, an obligate intracellular protozoan capable of infecting a wide range of warm-blooded animals and humans. Transmission commonly occurs through ingestion of contaminated food or water, exposure to infected cat faeces, or congenital transfer from mother to fetus during pregnancy. While many infected individuals remain asymptomatic, severe disease can develop in immunocompromised patients, transplant recipients, and pregnant women, where the infection may lead to neurological complications, ocular disease, or congenital abnormalities in newborns. Due to the non-specific clinical presentation and potential severity of complications, rapid and accurate laboratory diagnosis is essential for effective disease management and monitoring.
The Toxo-Q Real-Time PCR Kit from Genes2Me is a highly sensitive molecular assay designed for the qualitative detection of Toxoplasma gondii DNA in research samples including blood, urine, swab specimens, cerebrospinal fluid, and stool samples. Built on advanced real-time PCR technology, the assay delivers reliable pathogen detection with excellent specificity, supporting research and screening workflows in molecular laboratories.
The Toxo-Q Real-Time PCR Kit utilizes TaqMan-based multiplex real-time PCR chemistry for the detection of Toxoplasma gondii DNA. Specific primers and fluorescent probes target conserved regions of the parasite genome, enabling precise amplification and detection in the HEX fluorescence channel. The assay also incorporates an Internal Control labelled with Cy5 dye to monitor extraction quality, detect PCR inhibition, and validate amplification efficiency throughout every run.
The kit includes positive and no-template controls to ensure assay integrity and contamination-free operation. Ready-to-use reagents simplify laboratory workflow, minimise hands-on time, and reduce the risk of pipetting or preparation errors. The assay is compatible with multiple real-time PCR platforms including QuantStudio 5, Bio-Rad CFX96, LightCycler 480, and RapiCycler 96 systems.
Traditional serological methods may not always differentiate between active and past infection and can show limited sensitivity in immuno-compromised individuals. Molecular testing provides direct detection of Toxoplasma gondii DNA, enabling earlier and more accurate confirmation of infection. Real-time PCR offers rapid turnaround time, high analytical sensitivity, and superior specificity, making it particularly valuable in congenital screening, neurological infections, and immunocompromised patient management.
The Toxo-Q assay supports dependable detection even at low DNA concentrations and helps laboratories achieve reliable results for research and surveillance purposes. Integrated internal controls further strengthen confidence in assay performance and result interpretation.
High analytical sensitivity and specificity Delivers accurate qualitative detection of Toxoplasma gondii DNA, enabling reliable identification even in samples with low target concentrations.
The Cy5-channel Internal Control validates extraction quality and PCR efficiency in every run, reducing the risk of false-negative results caused by sample degradation or PCR inhibition.
Positive and no-template controls included Built-in assay controls ensure workflow integrity, contamination-free operation, and confidence in every test run.
Early molecular detection of active infection Direct detection of Toxoplasma gondii DNA enables earlier confirmation compared with serological methods, supporting research into congenital, neurological, and opportunistic infections.
Supports diverse sample types Validated for use with blood, urine, swab, cerebrospinal fluid (CSF), and stool specimens, providing flexibility across a broad range of research applications.
Optimized multiplex real-time PCR chemistry delivers consistent amplification and dependable qualitative results across different operators and laboratory workflows.
Ready-to-use reagents for streamlined workflow Pre-formulated reagents minimize hands-on time, reduce pipetting errors, and improve laboratory efficiency while ensuring consistent assay performance.
Compatible with multiple leading PCR platforms Validated on QuantStudio 5, Bio-Rad CFX96, LightCycler 480, and RapiCycler 96, enabling seamless integration into existing molecular laboratory workflows.
Toxo-Q Real Time PCR specific amplification plot of Toxoplasma Gondii Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| Toxo-Q Real-Time PCR Kit | G2M709621 | G2MBR4-0781 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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