Syphilis is a sexually transmitted infection caused by the bacterium Treponema pallidum. It is primarily transmitted through sexual contact and may also be passed from mother to fetus during pregnancy, leading to congenital Syphilis. The disease progresses through multiple stages, beginning with painless sores (chancres), followed by rash, fever, swollen lymph nodes, and potentially severe complications affecting the heart, brain, and nervous system if left untreated. Early and accurate detection is essential for effective treatment and prevention of complications.
The Syphilis-Q Real-Time PCR Kit from Genes2Me is a highly sensitive and specific in vitro diagnostic assay designed for the qualitative detection of Treponema pallidum DNA from clinical samples. The assay supports rapid molecular diagnosis and helps laboratories deliver reliable results for timely patient management.
The Syphilis-Q kit utilizes advanced real-time PCR technology with primer and probe sets specifically targeting conserved genes of Treponema pallidum. The assay employs HEX-labeled probes for pathogen detection and a Cy5-labeled internal control to validate sample quality, nucleic acid extraction, and PCR efficiency in every run. Positive and negative controls ensure workflow integrity and reliable assay performance.
The ready-to-use reagents and optimized workflow minimize hands-on time while reducing the risk of contamination and procedural errors.
Serological methods remain widely used for Syphilis screening; however, molecular detection offers enhanced sensitivity and specificity, particularly during early infection stages or in samples with low pathogen load. Real-time PCR enables direct detection of Treponema pallidum DNA, supporting earlier diagnosis and improved patient management.
The assay provides reliable results even in challenging clinical presentations and supports confirmation in cases where serological results may be inconclusive.
Advanced TaqMan-based real-time PCR technology enables highly sensitive and specific detection of Treponema pallidum DNA, ensuring reliable identification even in samples with low bacterial burden.
Direct detection of T. pallidum DNA supports diagnosis during early stages of infection when serological responses may be absent, delayed, or inconclusive, enabling timely clinical intervention.
A Cy5-labelled Internal Control validates nucleic acid extraction quality, sample integrity, and PCR amplification efficiency in every reaction, reducing the risk of false-negative results.
Built-in Positive Control and No Template Control (NTC) reactions verify assay integrity, monitor contamination, and ensure confidence in result interpretation throughout the testing workflow.
Provides clear qualitative reporting of Treponema pallidum DNA (Detected / Not Detected), simplifying result interpretation and supporting rapid clinical decision-making.
Pre-formulated reagents minimise hands-on preparation, reduce operator variability, and improve laboratory efficiency while lowering the risk of procedural errors.
Validated on Bio-Rad CFX96, QuantStudio™ 5, Roche LightCycler® 480, and RapiCycler 96 systems, allowing seamless integration into established molecular diagnostic workflows.
Facilitates early detection of maternal infection and supports efforts to reduce the risk of congenital syphilis through timely diagnosis and treatment.
Syphilis Real Time PCR specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)
| Commercial Name | Old Cat No. | New Cat No. | Pack Size |
|---|---|---|---|
| Syphilis-Q Real-Time PCR Kit | G2M705521 | G610048-1 | 50 Tests |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
© 2025 Genes2me. All rights reserved.
