Rota-Q Real Time PCR Kit for Rotavirus

Product Overview

Rotavirus is a highly contagious virus that belongs to the Sedoreoviridae family. It is one of the most common causes of severe diarrhoea in infants and young children worldwide, especially in developing countries. The virus is transmitted through the faecal-oral route, primarily through contaminated water and food, or direct contact with infected individuals, and it can survive on surfaces for several hours, making it very easy to spread. Symptoms of Rotavirus infection typically last for 3 to 8 days and, in most cases, the infection is self limiting. However, severe cases can lead to dehydration, electrolyte imbalances, and even death, especially in young children and immunocompromised individuals.

To address this important need, Genes2Me introduces the Rota-Q Real Time PCR Kit for Rotavirus, a screening test based on real time PCR technology for the detection of Rotavirus RNA in research samples. The assay is intended to be performed by a laboratory professional in a research laboratory.

Targets:
Rotavirus RNA
Technology:
Real-Time PCR (Multiplex)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Stool
Certification:
RUO (For Research Use Only)
Rota-Q Real Time PCR Kit for Rotavirus

How the Assay Works

The Rota-Q Kit is a highly reliable and accurate screening test for detecting all human Rotavirus. Utilizing real time PCR technology, the kit features fluorescent probes and specialized primers targeting Rotavirus sequences, tagged with a FAM fluorescent reporter, alongside an Internal Control labelled with HEX. Its multiplex design allows simultaneous detection of Rotavirus specific RNA and the Internal Control in separate detector channels, and it includes a positive control with a known sequence RNA template labelled with FAM to monitor amplification failure, ensuring precise and dependable results for diagnosing Rotavirus infections.

Clinical Relevance

Rotavirus is a highly contagious virus that causes severe diarrhoea, vomiting, fever, and abdominal pain, especially in infants and young children. There are several different strains of Rotavirus, classified based on the proteins on their outer surface. Currently, two oral vaccines are available to prevent Rotavirus infection, and the vaccine is highly effective in preventing severe Rotavirus disease in infants and young children. Rapid molecular screening for Rotavirus RNA supports timely identification of the causative agent in cases of severe paediatric diarrhoea, helping inform supportive care and broader public health surveillance efforts.

Sample Type & Reporting Output

Sample Type:
Stool
Reporting Output:
Qualitative detection of Rotavirus RNA (Detected / Not Detected)
Targets Detected:
Rotavirus specific RNA and Internal Control in a single multiplex reaction

Key Features & Benefits

Reliable Multiplex Detection

Simultaneous detection of Rotavirus specific RNA (FAM) and an Internal Control (HEX) in separate detector channels within a single reaction.

Integrated Internal Control

An Internal Control monitors amplification failure in every run, supporting confidence in qualitative result interpretation.

Stool Sample Compatibility

Designed for stool samples collected in a sterile container, supporting straightforward sample collection for Rotavirus screening.

Flexible Compatibility

Validated on leading Real-Time PCR platforms including Thermo Fisher QuantStudio 5, Bio-Rad CFX96, Roche LightCycler 480, and Genes2Me RapiCycler 96.

Long Shelf Life

The kit has a shelf life of 12 months from the date of manufacture and remains stable until the labelled expiration date when stored as specified.

Applications

  • Screening stool samples from infants and young children with severe diarrhoea for Rotavirus RNA in research settings
  • Supporting epidemiological surveillance studies on Rotavirus prevalence and strain distribution in developing and developed regions
  • Research into Rotavirus transmission patterns, seasonality, and the impact of oral vaccination programs
  • Investigational use in outbreak investigations involving severe paediatric gastroenteritis
  • Academic and public health research studying Rotavirus biology and disease burden

With the Rota-Q Real Time PCR Kit for Rotavirus, research laboratories can achieve sensitive, specific, and dependable screening for Rotavirus RNA in stool samples. By supporting rapid identification of this leading cause of severe paediatric diarrhoea, the kit helps researchers generate timely, informed insights for ongoing Rotavirus research and surveillance. Empower your laboratory with the Rota-Q Real Time PCR Kit, because rapid molecular insights can make a lasting difference in research outcomes.

Data Analysis and Report

EEC-Q Real Time PCR Kit for Enterovirus

Rota-Q Real Time PCR Kit for Rotavirus , specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
Rota-Q Real Time PCR Kit for Rotavirus G2M801621 G2M801621 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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