Nipah-Q Real-Time PCR Kit

Product Overview

Nipah virus (NiV) is a highly pathogenic zoonotic virus belonging to the Henipavirus genus of the Paramyxoviridae family. Fruit bats of the Pteropus species serve as the natural reservoir, with transmission occurring through direct contact with infected animals, contaminated food products, or infected individuals. Nipah virus infection can cause severe respiratory illness, acute encephalitis, and multi-organ complications, with reported mortality rates ranging from 40% to 75% depending on outbreak circumstances and healthcare access.

The Nipah-Q Real-Time PCR Kit from Genes2Me is a highly sensitive and specific in vitro diagnostic assay for the qualitative detection of Nipah virus RNA in human clinical specimens. The assay combines robust molecular detection with comprehensive quality controls, enabling reliable diagnosis during the early stages of infection when viral loads are highest and clinical intervention is most effective.

Targets:
Nipah Virus (NiV RNA)
Technology:
One-Step Real-Time RT-PCR (TaqMan)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Throat Swab, Nasopharyngeal Swab, Serum, Plasma, CSF
Certification:
CE-IVD
Nipah-Q

How the Assay Works

The Nipah-Q kit is a CE-IVD marked assay based on advanced real-time RT-PCR technology. The assay utilizes highly specific primers and probes targeting conserved regions of the Nipah virus genome, enabling sensitive and accurate detection of viral RNA.

A built-in Internal Control (HEX channel) monitors sample quality, nucleic acid extraction efficiency, and PCR performance in every reaction. Positive and Negative Controls supplied with the kit validate assay performance and help eliminate false positive and false negative results. The ready-to-use reagent format minimizes hands-on time, reduces contamination risks, and ensures consistent performance across laboratory workflows.

Why Molecular Testing Matters for Nipah Virus

Nipah virus infection presents with non-specific symptoms during the early phase, making clinical diagnosis difficult. Serological testing may not provide reliable results during acute infection due to delayed antibody production. Real-time RT-PCR remains the gold standard for early Nipah virus detection because it directly identifies viral RNA before seroconversion occurs. Early molecular confirmation supports:

  • Prompt patient isolation and infection control measures
  • Timely clinical intervention
  • Contact tracing and outbreak management
  • Accurate differentiation from other encephalitic and respiratory viral infections

Rapid molecular diagnosis is particularly important in regions experiencing outbreaks, where early detection can significantly reduce transmission and improve public health response.

Sample Type & Reporting Output

Sample Type:
Throat Swab, Nasopharyngeal Swab, Serum, Plasma, CSF
Reporting Output:
Qualitative Detection of Nipah Virus RNA (Detected / Not Detected)
Targets Detected:
Nipah Virus RNA

Key Features & Benefits

High Analytical Accuracy

Analytical sensitivity of up to 100% with excellent clinical specificity, enabling reliable detection even at low viral loads with a Ct cut-off of ≤35.

Robust Internal Control

HEX-channel internal control validates sample quality, extraction efficiency, and amplification performance in every run.

Positive and Negative Controls Included

Comprehensive assay controls ensure workflow reliability and provide confidence in result interpretation.

Early Detection of Active Infection

Detects Nipah viral RNA during the acute phase of infection, facilitating rapid clinical and public health decision-making.

Reproducible Performance

Precision studies demonstrate low assay variability with coefficient of variation below 3%, ensuring consistent results across runs and operators.

Ready-to-Use Reagents

Pre-formulated reagents reduce preparation time, simplify workflow, and minimize procedural errors.

Long Shelf Life

Stable for 12 months under recommended storage conditions, simplifying inventory management.

Broad Instrument Compatibility

Validated on major real-time PCR platforms including: || RapiCycler 96 || Bio-Rad CFX96 || QuantStudio™ 5 || LightCycler® 480

Applications

  • Clinical & Hospital Laboratories Supports rapid and accurate diagnosis of suspected Nipah virus infections in patients presenting with encephalitis or severe respiratory disease.
  • Public Health Laboratories: Facilitates outbreak investigation, surveillance, and epidemiological monitoring during Nipah virus outbreaks.
  • Emergency Response Programs: Provides rapid molecular confirmation for timely implementation of infection control measures and patient isolation.
  • Critical Care & Infectious Disease Units: Enables prompt diagnosis in severely ill patients where rapid intervention can improve clinical outcomes.
  • Reference Laboratories: Supports confirmatory testing and large-scale screening programs during outbreak situations.
  • One Health Surveillance Programs: Useful for integrated surveillance efforts monitoring zoonotic disease emergence and transmission dynamics.

Analytical Performance

  • High analytical sensitivity for Nipah virus RNA detection
  • Clinical specificity >98%
  • Ct cut-off ≤35 for positive result interpretation
  • Internal Control (HEX) validates sample quality and PCR efficiency
  • Positive and Negative Controls included
  • Precision with coefficient of variation <3%
  • Stable performance throughout 12-month shelf life under recommended storage conditions
  • No significant cross-reactivity observed with common respiratory or encephalitic viral pathogens

Data Analysis and Report

nipah-q Real Time PCR

NIPAH Q Real Time PCR target specific amplification plot of NIPAH Virus RNA (FAM Channel) positive sample.

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
Nipah-Q Real-Time PCR Kit G2M705921R G610011 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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