EEC-Q Real Time PCR Kit for Enterovirus

Product Overview

Hand, foot, and mouth disease is a contagious illness primarily caused by enteroviruses, which encompass 108 serotypes classified into four groups: A, B, C, and D. Among these, Enterovirus 71 (EV-A71) and Coxsackievirus A16 (CoxA16) are the most common causes. This disease predominantly affects children under five years old, presenting with characteristic symptoms such as blisters on the hands, feet, and mouth.

To address this important clinical need, Genes2Me introduces the EEC-Q Real Time PCR Kit, an in vitro diagnostic test based on real time PCR technology for the qualitative detection of Enterovirus, Enterovirus 71, and Coxsackievirus A16 in clinical samples. The assay is intended to be performed by a laboratory professional in a clinical laboratory.

Targets:
Enterovirus (Universal), Enterovirus 71, Coxsackievirus A16
Technology:
Real-Time PCR (Single-Tube Multiplex qRT-PCR)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Pharyngeal and Tonsillar Swab
Certification:
IVD
EEC-Q Real Time PCR Kit for Enterovirus

How the Assay Works

The EEC-Q Kit is a single tube multiplex diagnostic assay leveraging real time reverse transcription PCR (qRT-PCR) technology to simultaneously detect RNA sequences of Enterovirus (universal), Enterovirus 71 (EV71), and Coxsackievirus A16 (CoxA16) from human samples. The kit uses specific primers and fluorescently labeled probes: FAM for Enterovirus, Texas Red (ROX) for EV71, VIC (HEX) for CoxA16, and Cy5 for the Internal Control. This robust multiplex system ensures accurate and reliable detection of extracted pathogen RNA within a single reaction, streamlining workflows for clinical and epidemiological applications.

Clinical Relevance

In rare cases, hand, foot, and mouth disease can lead to severe complications like myocarditis, pulmonary oedema, or aseptic meningoencephalitis. While many individuals infected with non-polio enteroviruses remain asymptomatic or experience mild symptoms such as fever, cough, rash, mouth sores, or muscle aches, children with asthma may face a heightened risk of severe illness. As there is no specific treatment, rapid identification of the causative pathogen supports clinical management decisions, and prevention through regular handwashing with soap and water for at least 20 seconds remains the most effective way to reduce the risk of infection.

Sample Type & Reporting Output

Sample Type:
Posterior Pharyngeal Wall and Bilateral Tonsillar Swab
Reporting Output:
Qualitative detection of Enterovirus, Enterovirus 71, and Coxsackievirus A16 RNA (Detected / Not Detected)
Targets Detected:
Enterovirus (universal), Enterovirus 71, Coxsackievirus A16, and Internal Control in a single multiplex reaction

Key Features & Benefits

Single Tube Multiplex Detection

Simultaneous detection of Enterovirus (universal, FAM), Enterovirus 71 (Texas Red), Coxsackievirus A16 (HEX), and an Internal Control (Cy5) within a single reaction.

Integrated Internal Control

An Internal Control confirms sample integrity and PCR performance in every run, supporting confidence in qualitative result interpretation.

Targeted Sample Compatibility

Designed for posterior pharyngeal wall and bilateral tonsillar swab samples, supporting standardized sample collection for hand, foot, and mouth disease diagnosis.

Flexible Compatibility

Validated on leading Real-Time PCR platforms including Thermo Fisher QuantStudio 5, Bio-Rad CFX96, Roche LightCycler 480, and Genes2Me RapiCycler 96.

Long Shelf Life

The kit has a shelf life of 12 months from the date of manufacture and remains stable until the labeled expiration date when stored as specified.

Applications

  • Rapid identification of Enterovirus, Enterovirus 71, and Coxsackievirus A16 in children presenting with hand foot mouth disease symptoms
  • Supporting clinical decision making in pediatric settings, particularly for children with asthma who face a heightened risk of severe illness
  • Differentiating Enterovirus 71 and Coxsackievirus A16 infections, given their differing potential for severe complications such as myocarditis or aseptic meningoencephalitis
  • Epidemiological surveillance and outbreak investigation of hand foot mouth disease in clinical microbiology laboratories
  • Supporting infection control measures in childcare and school settings during hand foot mouth disease outbreaks

With the EEC-Q Real Time PCR Kit, laboratories can achieve sensitive, specific, and clinically meaningful detection of the major pathogens responsible for hand foot mouth disease. By supporting rapid pathogen identification within a single tube multiplex reaction, the kit helps clinicians make timely, informed decisions for patient care and disease management. Empower your laboratory with the EEC-Q Real Time PCR Kit, because rapid molecular insights can make a lasting difference in patient outcomes.

Data Analysis and Report

EEC-Q Real Time PCR Kit for Enterovirus

EEC-Q Real Time PCR Kit for Enterovirus, specific amplification plot of Treponema pallidum Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
EEC-Q Real Time PCR Kit for Enterovirus G2M801321 G2M801321 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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