EBV-Q Real-Time PCR Kit

Product Overview

Epstein-Barr Virus (EBV) is one of the most widespread human viruses globally and is primarily transmitted through saliva and is the causative agent of infectious mononucleosis. Many individuals may remain asymptomatic after infection, EBV can establish lifelong latency within B lymphocytes and may reactivate under certain conditions, particularly in immunocompromised individuals. Monitoring EBV infection and viral reactivation is therefore critical in clinical settings, especially for transplant recipients, oncology patients, and individuals with weakened immune systems.

The EBV-Q Real-Time PCR Kit from Genes2Me is a highly sensitive molecular diagnostic assay designed for the rapid detection of Epstein-Barr Virus Types 1 and 2 DNA in human clinical samples. Utilizing advanced Real-Time PCR technology, the assay provides accurate and reliable results that support early diagnosis, disease monitoring, and timely clinical intervention.

Targets:

Epstein-Barr Virus Types 1 & 2 DNA (EBV DNA)

Technology:

Real-Time PCR (TaqMan)

Detection:

Qualitative Detection of EBV DNA (Detected / Not Detected)

Sample Type:

Human Blood, Plasma, Serum, and CSF Samples

Certification:

CE-IVD

How the Assay Works

The EBV-Q Real-Time PCR Kit utilizes TaqMan-based Real-Time PCR technology for the qualitative detection of Epstein-Barr Virus DNA. The assay employs highly specific primers and fluorescent probes targeting conserved regions of the EBV genome to ensure accurate identification of both EBV Type 1 and Type 2.

The assay is configured as a single-tube multiplex reaction, enabling simultaneous detection of the target virus and an Internal Control. Amplified EBV DNA is detected through the Texas Red fluorescence channel, while the Internal Control is monitored through the HEX channel.

Why Molecular Testing Matters for EBV Detection

EBV infections may present with a wide range of clinical manifestations, from asymptomatic infection and infectious mononucleosis to severe complications in immunocompromised individuals. Because the virus can remain latent and reactivate periodically, accurate laboratory detection plays a vital role in patient management.

Conventional diagnostic approaches such as serological testing may not always distinguish active infection from previous exposure, particularly in patients with altered immune responses. Molecular diagnostics test such as Real-Time PCR provides a direct and highly specific, superior sensitive method for detecting even low viral DNA, enabling earlier and more reliable identification of active EBV infection.

By providing reliable detection of EBV DNA, the EBV-Q Real-Time PCR Kit helps clinicians make informed treatment decisions and improve patient outcomes.

Key Features & Benefits

High Analytical Performance

Combines excellent sensitivity, specificity, and reproducibility for dependable diagnostic outcomes.

Built-In Process Control

Integrated Internal Control enhances result reliability by monitoring assay integrity throughout the workflow.

No Cross-Reactivity

Engineered for specific EBV detection without interference from common viral pathogens.

Wide Dynamic Detection Range

Supports reliable detection across a broad concentration range with excellent assay linearity.

Laboratory-Friendly Workflow

Simple assay setup, rapid amplification protocol, and straightforward result interpretation improve laboratory efficiency.

Applications

Clinical & Hospital Laboratories: Supports rapid diagnosis of EBV infections in patients presenting with infectious mononucleosis-like symptoms and related clinical manifestations.
Transplant Monitoring: Enables routine monitoring of transplant recipients who are at increased risk of EBV reactivation and post-transplant lymphoproliferative disorders.
Oncology & Hematology Centers: Assists in the detection and monitoring of EBV-associated malignancies including lymphomas and nasopharyngeal carcinoma.
Immunocompromised Patient Management: Provides reliable detection of EBV reactivation in patients with weakened immune systems, including oncology and immunosuppressed populations.
Research & Epidemiological Studies: Supports studies investigating EBV prevalence, viral reactivation, disease progression, and virus-associated cancers.

Analytical Performance

Analytical sensitivity of 100% for EBV DNA detection and analytical specificity of 100% with no observed cross-reactivity.
Clinical sensitivity of 96% and clinical specificity of 99%.
Limit of Detection (LOD) with 1100 copies/mL.
Linearity demonstrated with R² value of 0.997.
Ct cut-off value of ≤35 for positive result interpretation.
No cross-reactivity observed with HSV-1, HSV-2, Cytomegalovirus (CMV), or HIV.
Internal Control validates sample quality and assay performance in every reaction.
Shelf life of 12 months when stored under recommended conditions.

Commercial Name	Old Cat No.	New Cat No.	Pack Size
EBV-Q Real Time PCR Kit	G2M705621	G2M705621	50 Tests

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Product Overview

How the Assay Works

Why Molecular Testing Matters for EBV Detection

Sample Type & Reporting Output