C. Albicans-Q Real-Time PCR Kit

Product Overview

Candida albicans is the most common opportunistic fungal pathogen responsible for approximately 70% of human fungal infections. While it normally exists as part of the human microbiota, it can cause superficial infections such as oral thrush and vulvovaginal candidiasis, as well as life-threatening invasive candidiasis in immunocompromised and critically ill patients.

The Genes2Me C. Albicans-Q Real-Time PCR Kit is a highly sensitive in vitro diagnostic assay designed for the qualitative detection of Candida albicans DNA in clinical samples. Utilizing advanced TaqMan real-time PCR technology with integrated internal controls, the assay enables rapid, accurate, and reliable molecular diagnosis, facilitating timely clinical intervention and improved patient outcomes.

Targets:
Candida albicans DNA
Technology:
Real-Time PCR (TaqMan)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Blood, Genital Swabs, Clinical Fluids
Certification:
CE-IVD
C. Albicans-Q

How the Assay Works

The C. Albicans-Q Real-Time PCR Kit employs TaqMan real-time PCR technology with species-specific primers and fluorescent probes targeting Candida albicans DNA. Detection is performed through the FAM channel, while the RNaseP internal control, detected in the Cy5 channel, confirms successful nucleic acid extraction and PCR amplification.

A positive control validates assay performance and reagent integrity, while the no-template control monitors contamination. The optimized ready-to-use workflow minimizes hands-on time and ensures consistent laboratory performance.

Why Molecular Testing Matters for Candida albicans

Conventional culture methods for fungal detection often require several days and may demonstrate limited sensitivity, delaying appropriate antifungal therapy. Real-time PCR enables rapid and highly specific detection of Candida albicans DNA directly from clinical specimens, significantly reducing turnaround time while improving diagnostic accuracy.:

Early molecular detection is particularly critical for immunocompromised patients and invasive candidiasis, where prompt diagnosis and treatment are associated with improved survival and reduced healthcare burden.

Sample Type & Reporting Output

Sample Type:
Blood, Genital Swabs, Clinical Fluids
Reporting Output:
Qualitative detection (Detected / Not Detected)
Targets Detected:
Candida albicans DNA

Key Features & Benefits

High analytical sensitivity and specificity

High analytical sensitivity and specificity enabling accurate detection of Candida albicans DNA with a Ct cut-off of ≤35.

Built-in RNaseP internal control

Built-in RNaseP internal control validates nucleic acid extraction quality and PCR amplification, minimizing false-negative results.

Positive and Negative Controls Included

Positive and no-template controls included for comprehensive assay validation and contamination monitoring.

Rapid molecular detection

Rapid molecular detection supporting early diagnosis compared to conventional culture-based methods.

Highly specific TaqMan

Highly specific TaqMan chemistry designed for reliable identification of Candida albicans.

Ready-to-Use Reagents

Ready-to-use reagents with 12-month shelf life, simplifying workflow and inventory management.

Compatible with multiple leading PCR platforms

Compatible with multiple leading PCR platforms, including RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480.

Simple standardized workflow

Simple standardized workflow suitable for routine clinical molecular laboratories.

Applications

  • Clinical & Hospital Laboratories: Provides rapid molecular detection of Candida albicans from clinical specimens, supporting timely diagnosis and effective patient management.
  • Critical Care & Intensive Care Units: Facilitates early detection of invasive candidiasis in critically ill patients, enabling prompt antifungal therapy.
  • Immunocompromised Patient Management: Supports diagnosis in transplant recipients, oncology patients, HIV patients, and other immunosuppressed individuals at high risk for invasive fungal infections.
  • Women's Health Laboratories: Enables accurate detection of Candida albicans associated with vulvovaginal candidiasis and recurrent fungal infections.
  • Infection Control & Hospital Surveillance: Assists healthcare facilities in monitoring fungal infections and reducing healthcare-associated infections through rapid molecular testing.
  • Reference & Molecular Diagnostic Laboratories: Provides standardized, high-performance PCR testing for routine fungal diagnostics and confirmatory testing.

Analytical Performance

  • Qualitative detection of Candida albicans DNA using Real-Time PCR technology.
  • Ct cut-off ≤35 for positive sample interpretation.
  • RNaseP internal control (Cy5) validates nucleic acid extraction and PCR performance.
  • Positive and no-template controls included to ensure assay integrity and contamination-free testing.
  • Compatible with major Real-Time PCR platforms, supporting seamless laboratory integration.
  • 12-month shelf life under recommended storage conditions.
  • Rapid, sensitive, and specific molecular detection suitable for routine clinical diagnostics and early infection management.

Data Analysis and Report

c.albicans Real Time PCR

C.Albicans Q Real Time PCR target specific amplification plot of Candida albicans DNA (FAM Channel) positive sample.

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
C. Albicans-Q Real-Time PCR Kit G2M708021R G2M708021 50 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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