YCM-Q Real Time PCR Kit

Product Overview

Male infertility affects millions of couples worldwide, and genetic factors are among the most significant underlying causes. Y chromosome microdeletions (YCM) are recognized as one of the most common genetic causes of impaired spermatogenesis, contributing to azoospermia and severe oligozoospermia. These deletions occur within specific regions of the long arm of the Y chromosome (Yq11), known as the Azoospermia Factor (AZF) regions, which are critical for normal sperm production.

The YCM-Q Real Time PCR Kit from Genes2Me is an advanced in vitro diagnostic assay designed for the qualitative detection of microdeletions across 15 clinically relevant loci on the human Y chromosome. Utilizing highly specific real-time PCR technology, the assay enables rapid and reliable screening of AZFa, AZFb, AZFc, and AZFd regions from DNA extracted from EDTA blood samples.

By providing comprehensive coverage of the major Y chromosome deletion hotspots associated with male infertility, the YCM-Q kit supports clinicians and fertility specialists in identifying genetic causes of spermatogenic failure and guiding informed reproductive counselling and treatment decisions.

Targets:
15 Y-Chromosome Microdeletion Markers (AZFa, AZFb, AZFc, AZFd Regions)
Technology:
Real-Time PCR (TaqMan)
Detection:
Qualitative detection of Y Chromosome Microdeletions (Detected / Not Detected)
Sample Type:
Whole blood samples
Certification:
CE-IVD
YCM-Q

How the Assay Works

The YCM-Q Real Time PCR Kit utilizes probe-based real-time PCR technology to detect the presence or absence of 15 key sequence-tagged sites (STS) across the Y chromosome.

The assay targets:

  • Control Regions: SY14 and ZFY
  • AZFa Region: SY84, SY86, SY81
  • AZFb Region: SY127, SY134, SY164, SY142
  • AZFc/DAZ Region: SY255, SY254, SY277
  • AZFc/d Region: SY152, SY145, SY153

The kit contains five ready-to-use reaction mixes, each designed to detect three target loci along with a Beta-Actin internal control. During amplification, target-specific fluorescent probes generate signals that are monitored in real time through FAM, HEX/VIC, Texas Red/ROX, and Cy5 channels. Amplification of a target indicates the presence of that genomic region, while absence of amplification suggests a potential microdeletion. Built-in internal controls, positive controls, and no-template controls ensure assay integrity, sample quality verification, and contamination-free testing.

Why Molecular Testing Matters for Y Chromosome Microdeletions

Y chromosome microdeletions cannot be identified through routine semen analysis or standard clinical examinations. Molecular testing is therefore essential for accurate diagnosis of genetic infertility.

Microdeletions within AZF regions are strongly associated with impaired sperm production and may significantly influence fertility treatment outcomes. Early identification helps clinicians determine the likelihood of successful sperm retrieval procedures, select appropriate assisted reproductive technologies, and provide accurate genetic counselling to affected individuals and couples.

Sample Type & Reporting Output

Sample Type:
Whole blood samples
Reporting Output:
Qualitative detection of Y Chromosome Microdeletions
Targets Detected:
15 Y-Chromosome Microdeletion Markers (AZFa, AZFb, AZFc, AZFd Regions)

Key Features & Benefits

Comprehensive Y chromosome coverage

Detects 15 clinically relevant loci across AZFa, AZFb, AZFc, and AZFd regions, providing extensive screening for male infertility-associated microdeletions.

Built-in internal control

Beta-Actin internal control in every reaction validates DNA quality, extraction efficiency, and PCR performance.

Ready-to-use master mixes

Pre- formulated reaction mixes reduce hands-on time, minimize preparation errors, and improve workflow efficiency.

Positive and negative controls included

Comprehensive assay controls help validate test performance and ensure confidence in every run.

Broad instrument compatibility

Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480 platforms.

Applications

  • Male Infertility Evaluation: Supports identification of genetic causes of azoospermia and severe oligozoospermia by detecting clinically relevant Y chromosome microdeletions.
  • Fertility Clinics and IVF Centers: Assists reproductive specialists in selecting appropriate assisted reproductive techniques and predicting sperm retrieval outcomes.
  • Genetic Counseling Programs: Enables informed counseling regarding inheritance risks, fertility prognosis, and reproductive planning.
  • Pre-ART Assessment: Useful before assisted reproductive technology (ART) procedures to identify genetic abnormalities that may affect treatment success.
  • Clinical Research Studies: Supports research focused on genetic determinants of male infertility and spermatogenic failure.

Analytical Performance

  • Detection of 15 clinically significant Y chromosome loci covering AZFa, AZFb, AZFc, and AZFd regions
  • Internal control (Beta-Actin, Cy5 channel) validates DNA quality and PCR performance in every reaction
  • Ct cut-off value of ≤33 for target detection
  • Positive control Ct value ≤28 for assay validation
  • Internal control Ct range of 22–26 confirms acceptable assay performance
  • Ready-to-use master mixes minimize variability and improve assay consistency
  • Designed for qualitative identification of Y chromosome microdeletions associated with male infertility

Data Analysis and Report

YCM-Q Real Time PCR

YCM-Q Real Time PCR specific amplification plot of micro deletions in human Y chromosome along with internal control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
YCM-Q Real Time PCR Kit G2M803021 G610033 25 Tests

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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