Thrombosis-Q Comprehensive Real Time PCR Kit

Product Overview

Thrombosis is a condition characterized by the formation of blood clots within blood vessels, which can result in blood flow restriction and lead to serious health complications such as deep vein thrombosis (DVT), pulmonary embolism (PE), stroke, and pregnancy-related disorders. Other factors such as several environmental and lifestyle factors influence thrombotic risk, inherited genetic variants are recognized as important contributors to abnormal clot formation.

Inherited thrombophilia refers to a genetic predisposition that increases an individual's likelihood of developing thrombotic events. Identifying these genetic risk factors plays an important role in clinical risk assessment, preventive management, genetic counselling, and long-term patient care.

The Thrombosis-Q Comprehensive Real-Time PCR Kit is an in vitro diagnostic assay developed for the qualitative detection of clinically relevant thrombosis-associated genetic variants. Utilizing advanced real-time PCR technology, the assay enables laboratories to identify major inherited thrombophilia markers in a single workflow, supporting accurate genotype determination and confident clinical decision-making.

Targets:

Factor II (FII) G20210A (Prothrombin), Factor V (FV) Leiden G1691A, Factor V 1299 (H1299R/HR2), MTHFR C677T, MTHFR A1298C, and PAI-1 4G/5G polymorphism

Technology:

Real-Time PCR (TaqMan)

Detection:

Qualitative (Detected / Not Detected)

Sample Type:

Whole blood samples

Certification:

CE-IVD

How the Assay Works

The Thrombosis-Q Comprehensive Real-Time PCR Kit allows allele-specific real-time PCR technology to detect genetic variants associated with inherited thrombophilia. The assay uses mutation-specific primers and fluorescent probes that selectively amplify and differentiate wild-type and mutant alleles present in DNA extracted from whole blood samples.

The kit is designed to detect:

Factor II (Prothrombin) G20210A mutation
Factor V Leiden G1691A mutation
MTHFR C677T mutation
MTHFR A1298C mutation
Factor V H1299R polymorphism
PAI-1 4G/5G polymorphism

Four ready-to-use reaction mixes allow detection of these thrombosis-associated genetic variants across FAM, HEX, Texas Red and Cy5 channels. An internal control validates DNA quality and amplification efficiency in every reaction, while positive and negative controls ensure assay integrity and reliable result interpretation. The assay provides qualitative genotype identification and enables differentiation between wild-type, heterozygous mutant, and homozygous mutant genotypes.

Why Molecular Testing Matters for Thrombosis

Inherited thrombophilia-associated mutations may remain undetected for years until triggered by additional risk factors such as surgery, prolonged immobilization, pregnancy, hormonal therapy, obesity, or malignancy. Early identification of genetic susceptibility enables clinicians to evaluate thrombotic risk more effectively and implement appropriate preventive strategies when required.

Unlike conventional coagulation assays, which evaluate functional clotting activity, molecular testing directly identifies inherited genetic variants associated with thrombosis risk. This provides definitive genetic information that can support comprehensive thrombophilia assessment, patient stratification, family screening, and personalized healthcare decisions.

Key Features & Benefits

Wide mutation coverage

Comprehensive thrombophilia mutation panel covering major inherited risk markers.

Precise Genotype Identification

Accurate differentiation of wild-type, heterozygous and homozygous mutant genotypes

Built-In Process Control

Internal control in every reaction to verify DNA quality and PCR performance.

Assay Validation Assurance

Positive and negative controls included for workflow validation.

Multi-Platform Compatibility

Compatible with leading real-time PCR platforms including QuantStudio 5, Bio-Rad CFX96, LightCycler 480 and RapiCycler 96.

Clinically Actionable Insights

Supports evidence-based thrombophilia risk assessment and genetic counselling.

Applications

Clinical & Hospital Laboratories: Detection of inherited thrombophilia-associated mutations.
Haematology & Coagulation Testing Laboratories: Supports thrombotic risk assessment and patient stratification.
Preventive & Personalized Medicine: Identification of individuals with genetic predisposition to thrombosis.
Family Screening Programs: Evaluation of inherited thrombophilia within affected families.
Research & Genetic Epidemiology Studies: Investigation of thrombophilia-associated polymorphisms across populations.
Reproductive Health & Pregnancy Risk Assessment: Supports assessment of thrombosis-related genetic risk factors.

Analytical Performance

Qualitative detection of clinically relevant thrombophilia-associated genetic mutations.
Distinguishes wild-type, heterozygous and homozygous mutant genotypes.
Internal control validates assay performance in every reaction.
Positive and negative controls support contamination-free result verification.
Validated real-time PCR workflow for reliable genotyping.
Compatible with multiple industry-standard PCR platforms.
Stable for 12 months under recommended storage conditions.

Commercial Name	Old Cat No.	New Cat No.	Pack Size
Thrombosis-Q Comprehensive Real Time PCR Kit	G2M803421	G610037	50 Tests

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

Product Overview

How the Assay Works

Why Molecular Testing Matters for Thrombosis

Sample Type & Reporting Output