HIV-Q Real-Time PCR Kit

Product Overview

Human Immunodeficiency Virus (HIV) is a retrovirus that attacks the body's immune system, specifically CD4+ T lymphocytes, leading to progressive immune suppression and increased susceptibility to opportunistic infections and certain cancers. Monitoring HIV viral load is a critical component of patient management, helping assess disease progression, treatment response, and antiretroviral therapy efficacy.

The HIV-Q Real-Time PCR Kit from Genes2Me is a highly sensitive and specific molecular assay designed for the detection and quantification of HIV-1 RNA in human plasma samples. The assay delivers accurate viral load measurements, enabling researchers and laboratories to monitor viral replication dynamics with confidence and precision.

Targets:
Human Immunodeficiency Virus Type 1 (HIV-1 RNA)
Technology:
One-Step Quantitative Real-Time RT-PCR (RT-qPCR)
Detection:
Quantitative (Viral Load in Copies/mL and IU/mL)
Sample Type:
Human Plasma
Certification:
Research Use Only (RUO)
HIV-Q RT-PCR Kit

How the Assay Works

The HIV-Q assay utilizes quantitative Reverse Transcription Real-Time PCR (RT-qPCR) technology to amplify and quantify conserved regions of the HIV-1 genome. HIV-specific amplification is detected through the HEX fluorescence channel, while an Internal Control detected in the Cy5 channel monitors extraction efficiency, amplification performance, and potential PCR inhibition.

The kit includes four Quantification Standards (QS1–QS4) with defined copy numbers, allowing generation of a standard curve for precise viral load calculation. The assay is compatible with both open and closed real-time PCR platforms and provides reliable quantitative results across a broad dynamic range.

Why Molecular Viral Load Testing Matters

Accurate HIV viral load monitoring is essential for evaluating treatment effectiveness and identifying therapeutic failure before clinical symptoms appear. Molecular testing directly quantifies circulating HIV RNA, providing a sensitive measure of active viral replication. Regular viral load assessment supports:

  • Monitoring antiretroviral therapy response
  • Early detection of virological failure
  • Assessment of treatment adherence
  • Longitudinal disease management
  • Clinical research and epidemiological studies

Sample Type & Reporting Output

Sample Type:
Human Plasma
Reporting Output:
Quantitative HIV-1 RNA Viral Load
Targets Detected:
HIV-1 RNA
Detection Channels:
HEX (HIV), Cy5 (Internal Control)

Key Features & Benefits

Quantitative viral load measurement

Quantitative viral load measurement Enables accurate HIV-1 RNA quantification in copies/mL and IU/mL for monitoring viral replication and treatment response.

High analytical performance

High analytical performance Demonstrates 100% analytical sensitivity and specificity with reliable detection across a broad dynamic range.

Low limit of detection

Low limit of detection Detects as low as 40 copies/mL (25 IU/mL), supporting sensitive identification of low-level viremia.

Built-in internal control

Built-in internal control Cy5-channel internal control validates extraction quality, amplification efficiency, and assay performance in every reaction.

Four quantification standards included

Four quantification standards included Predefined standards facilitate robust standard curve generation for accurate viral load determination.

Excellent reproducibility

Excellent reproducibility Precision studies demonstrate coefficient of variation below 3%, ensuring consistent performance across runs.

Broad instrument compatibility

Broad instrument compatibility Validated on QuantStudio™ 5, Bio-Rad CFX96, Roche LightCycler® 480, and Genes2Me RapiCycler 96 platforms.

Stable ready-to-use reagents

Stable ready-to-use reagents Pre-formulated reagents with 12-month shelf life simplify workflow and minimize hands-on preparation.

Applications

  • Clinical Research Laboratories: Supports quantitative monitoring of HIV viral replication and evaluation of therapeutic interventions in research settings.
  • Antiretroviral Therapy Monitoring: Provides accurate viral load measurements for assessment of treatment efficacy and virological response.
  • HIV Disease Progression Studies: Enables longitudinal monitoring of viral dynamics and disease progression in clinical research programs.
  • Pharmaceutical & Drug Development Research: Facilitates evaluation of investigational antiviral therapies and therapeutic monitoring studies.
  • Epidemiological & Public Health Research: Supports surveillance programs and population-based HIV research initiatives.
  • Academic & Translational Research: Provides reliable quantitative molecular data for HIV pathogenesis and virology studies.

Analytical Performance

  • Analytical sensitivity & specificity: 100%
  • Clinical accuracy: 99%
  • Limit of Detection (LOD): 40 copies/mL (25 IU/mL)
  • Measuring range: 10–10⁹ copies/reaction
  • Quantification standard slope: -3.24
  • Precision (Repeatability): CV < 3%
  • Precision (Reproducibility): CV < 3%
  • No cross-reactivity observed with HCV, HBV, and HSV
  • 12-month shelf life under recommended storage conditions

Data Analysis and Report

HIV-Q Real Time PCR Kit

HIV-Q Real Time PCR specific amplification plot of HIV Positive Control (HEX Channel) along with Internal Control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
HIV-Q Real Time PCR Kit G2MBR4-0077 50 Tests

Product Enquiry Form

Other Products

Rapi-Q Series
Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

Rapi-X96
RAPiCycler-96

Real time PCR system to process 96 samples.
TRapi-X96
Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

© 2026 Genes2me. All rights reserved.