3HDtect Real-Time PCR Kit

Product Overview

Human Immunodeficiency Virus (HIV), Hepatitis B Virus (HBV), and Hepatitis C Virus (HCV) are among the most significant blood-borne viral pathogens worldwide. Co-infection is common due to shared routes of transmission, including exposure to infected blood, unsafe injections, and sexual contact. Early and accurate detection is critical for patient management, epidemiological surveillance, and laboratory research.

The 3HDtect Real-Time PCR Kit from Genes2Me is a highly sensitive multiplex molecular assay designed for the simultaneous qualitative detection and differentiation of HIV RNA, HCV RNA, and HBV DNA from blood, serum, and plasma samples. The assay combines robust target-specific amplification with internal quality controls, enabling reliable identification of all three viral pathogens in a single reaction.

Targets:
HIV RNA, HCV RNA, HBV DNA
Technology:
Multiplex One-Step Real-Time RT-PCR (TaqMan)
Detection:
Qualitative (Detected / Not Detected)
Sample Type:
Blood, Serum, Plasma
Certification:
RUO (Research Use Only)
3HDtect-Q

How the Assay Works

The 3HDtect assay utilizes multiplex Real-Time RT-PCR technology incorporating pathogen-specific primers and fluorescent probes for HIV, HCV, and HBV detection.

  • HCV detection: FAM channel
  • HIV detection: HEX channel
  • HBV detection: Texas Red channel
  • Internal Control (IC): Cy5 channel

Following nucleic acid extraction, target sequences are amplified and detected in real-time. The Internal Control validates extraction efficiency and PCR performance, while Positive and No Template Controls ensure assay reliability and contamination monitoring.

Why Molecular Testing Matters

Conventional serological testing may fail to identify infections during the window period before antibody development. Molecular detection directly identifies viral nucleic acids, enabling earlier detection of infection and improved diagnostic confidence. The 3HDtect assay supports:

  • Early infection detection
  • Simultaneous screening for HIV, HBV, and HCV
  • Improved laboratory workflow efficiency
  • Reduced turnaround time through multiplex testing
  • Reliable differentiation of co-infections

Sample Type & Reporting Output

Sample Type:
Blood, Serum, Plasma
Reporting Output:
(Detected / Not Detected)
Targets Detected:
HIV RNA, HCV RNA, HBV DNA

Key Features & Benefits

Multiplex Detection in a Single Reaction

Simultaneously detects HIV, HCV, and HBV, reducing testing time and reagent consumption.

High Analytical Sensitivity

Detects low viral loads with LOD of 40 copies/mL for HIV, 50 copies/mL for HCV, and 70 copies/mL for HBV.

Robust internal control

Cy5-channel internal control validates nucleic acid extraction and amplification efficiency in every run.

Excellent Accuracy

Delivers 99% overall accuracy with high analytical sensitivity and specificity.

No Cross-Reactivity

Validated against HSV-1, HSV-2, CMV, EBV, and VZV with no observed cross-reactivity.

Highly Reproducible Results

Precision studies demonstrate CV below 2.5% for repeatability and reproducibility.

Broad Dynamic Range

Linear quantification performance across 10 to 10⁹ copies per reaction.

Compatible with Leading PCR Platforms

Validated on RapiCycler 96, Bio-Rad CFX96, QuantStudio 5, and LightCycler 480 systems.

Applications

  • Clinical & Research Laboratories: Supports molecular screening and research applications involving HIV, HBV, and HCV detection.
  • Blood Bank Screening Research: Facilitates evaluation of blood-borne viral contamination in research workflows.
  • Co-Infection Studies: Enables simultaneous detection of HIV-HBV and HIV-HCV co-infections.
  • Epidemiological Surveillance: Supports monitoring and research of viral prevalence and transmission patterns.
  • Academic & Translational Research: Useful for virology studies, assay development, and infectious disease investigations.
  • Public Health Research Programs: Provides reliable molecular detection for population-level screening studies.

Analytical Performance

  • Analytical Sensitivity & Specificity: 100%
  • Accuracy: 99%
  • Precision (Reproducibility): CV < 2.5%
  • Linearity: R² = 0.998
  • HIV LOD: 40 copies/mL (23.52 IU/mL)
  • HCV LOD: 50 copies/mL (13.66 IU/mL)
  • HBV LOD: 70 copies/mL (12.5 IU/mL)
  • Measuring Range: 10–10⁹ copies/reaction
  • No cross-reactivity with HSV-1, HSV-2, CMV, EBV, and VZV
  • Shelf Life: 12 months under recommended storage conditions

Data Analysis and Report

3HDTECT-Q Real Time PCR

3HDtect Real Time PCR specific amplification plot of HIV Positive Control (HEX Channel), HBV Positive Control (TEXAS RED Channel), & HCV Positive Control (FAM Channel) along with Internal Control (Cy5 Channel)

Ordering Information

Commercial Name Old Cat No. New Cat No. Pack Size
3HDtect Real Time PCR Kit G2MBR4-0666 50 Tests

Product Enquiry Form

Other Products

Rapi-Q Series
Rapi-Q

Rapid and Compact Point-of-care RT-PCR Testing Solution.

Rapi-X96
RAPiCycler-96

Real time PCR system to process 96 samples.
TRapi-X96
Rapi-X16

Compact Nucleic Acid Extraction System Ideal for Point-of-Care Testing

Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

© 2026 Genes2me. All rights reserved.