SpiNXT Fungal DNA Extraction Kit

SpiNXT Fungal DNA Extraction Kit

The SpiNXT Fungal DNA Extraction Kit is designed for the efficient isolation of high-quality genomic DNA from a wide range of fungal samples, including yeast cells and filamentous fungi. The kit enables rapid processing of fungal tissues with robust cell walls, delivering pure and intact DNA suitable for diverse downstream molecular biology applications.

Kit components: Buffer LBF-1 | Buffer LBF-2 | RNase A | Buffer W1 | Buffer W2 | Buffer AE

Blood DNA Extraction

Overview


The SpiNXT Fungal DNA Extraction Kit is based on silica membrane spin-column technology for reliable purification of fungal genomic DNA. The protocol employs optimized lysis buffers and enzymatic treatment to effectively disrupt tough fungal cell walls and release nucleic acids. RNase treatment ensures removal of RNA contaminants, allowing isolation of RNA-free genomic DNA. Following lysis, DNA binds selectively to the silica membrane under high-salt conditions. Impurities such as proteins, polysaccharides, and secondary metabolites are removed through successive wash steps. The purified DNA is finally eluted in a low-salt buffer, yielding high-purity fungal genomic DNA compatible with sensitive downstream applications.

Product Features


Blood DNA Extraction Kits
  • Recommended Input Amount: 50 mg/200 μl
  • Binding Mechanism: 30-40μg
  • Elution Volume: ≥ 30 µl
  • Purity: A260/280 -1.8±0.1
  • Expected Yield: Upto 30μg

Applications


Polymerase Chain Reaction (PCR)
  • Amplification of fungal genomic targets
  • Species identification and diagnostic assays
Quantitative Real-Time PCR (qPCR)
  • Gene expression and fungal load analysis
  • Pathogen detection and quantification
Restriction Enzyme Digestion
  • Genomic DNA digestion for molecular analysis
  • Genetic diversity and strain differentiation
Microbial and Mycological Research
  • Functional genomics and comparative studies
  • Industrial, agricultural, and environmental applications
ITS and rDNA region analysis
  • Environmental and clinical fungal studies
DNA Sequencing
  • Fungal genome characterization, Taxonomic and phylogenetic studies
  • Molecular Identification of Fungi

Panel Specifications


Format: Mini spin column
Technology: Silica membrane
Sample Type: 50 mg/200 μl Sample
Yield: Up to 30 µg
Target: Fungal DNA
A260/280: 1.6–1.9
Available Pack Sizes: 50 T, 250 T
Throughput Compatibility: Manual

Workflow / How It Works


Extraction workflow

Data Analysis and Reporting


SpiNXT Fungal DNA Extraction
Fungal DNA Extraction
Data/Report
Data/Report

Ordering Information


Commercial Name Cat No. Pack Size
SpiNXT Fungal DNA Extraction Kit G2MBR4-0810 50 T
SpiNXT Fungal DNA Extraction Kit G2MBR4-0813 250 T

Product Enquiry Form


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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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