SpiNXT Fecal RNA Extraction Kit

SpiNXT Fecal RNA Extraction Kit

The SpiNXT Fecal RNA Extraction Kit is designed for rapid and efficient isolation of high-quality total RNA from fresh or frozen fecal (stool) samples. The kit enables reliable recovery of RNA from complex fecal matrices containing host cells and diverse microorganisms, making it suitable for downstream molecular and clinical research applications.

Kit components: Buffer-FRLB | Carrier RNA | Proteinase K | Buffer BW2 | Proteinase K | Proteinase Dissolving Buffer | Wash Buffer 1 | Wash Buffer 2 | Buffer EB

Blood DNA Extraction

Overview


The SpiNXT Fecal RNA Extraction Kit is based on silica membrane spin-column technology, enabling selective binding of RNA under optimized lysis and binding conditions. Fecal samples are first homogenized and lysed to release RNA, followed by adsorption of RNA onto a silica membrane. Contaminants such as proteins, salts, inhibitors, and other fecal impurities are efficiently removed through wash steps. Purified RNA is finally eluted in a low-salt buffer or water, yielding high-integrity RNA suitable for sensitive downstream applications. The workflow eliminates the use of hazardous organic solvents and supports consistent, reproducible RNA purification from challenging fecal samples.

Product Features


Blood DNA Extraction Kits
  • Binding Capacity: 20-30µg genomic RNA
  • Recommended Input Amount: 100-150mg of stool sample
  • Elution Volume: ≥ 30 µl
  • Purity: A260/280 - 1.8±0.1, A260/230 - 2.0±0.1
  • Expected Yield: Depends upon the quality & quantity of the starting material used

Applications


Gut Microbiome Gene Expression Studies
  • Isolation of RNA for transcriptomic and functional analysis of microbial communities present in fecal samples.
RT-PCR and qRT-PCR Analysis
  • Purified RNA suitable for reverse transcription and quantitative gene expression studies of host and microbial targets.
Metatranscriptomic Analysis
  • Preparation of high-quality RNA for sequencing-based analysis of actively expressed genes within gut microbiota.
Infectious Disease Research
  • RNA extraction for detection and study of RNA-based pathogens and microbial gene expression in stool samples.
Clinical and Translational Research
  • Applicable in biomarker discovery, gastrointestinal disease research, and host–microbe interaction studies.

Panel Specifications


Format: Mini spin column
Technology: Silica membrane
Sample Type: Stool Sample
Yield: Depends upon the quality & quantity of the starting material used
Target: Genomic RNA, Fecal RNA
A260/280: 1.6 - 1.9
Available Pack Sizes: 50 / 250 T
Throughput Compatibility: Manual

Workflow / How It Works


Extraction workflow

Data Analysis and Reporting


SpiNXT Fecal RNA Extraction
Fecal RNA Extraction
Data/Report
Data/Report

Ordering Information


Commercial Name Cat No. Pack Size
SpiNXT Fecal RNA Extraction Kit G2MBR4-0719 50 T
SpiNXT Fecal RNA Extraction Kit G2MBR4-0720 250 T

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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