The SpiNXT Fecal DNA Extraction Kit is a robust and reliable solution for the isolation of high-quality genomic DNA from fresh or frozen stool (fecal) samples. The kit is optimized to efficiently recover DNA from complex fecal matrices, enabling simultaneous extraction of host DNA and microbial DNA for advanced molecular and microbiome studies. Its streamlined workflow ensures consistent performance while minimizing inhibitor carryover commonly associated with fecal samples.
Kit components: Buffer FLB-1 | Buffer FLB-2 | Buffer FLB-3 | Binding Buffer | Buffer TE | Proteinase K | Proteinase Dissolving Buffer | Wash Buffer 1 | Buffer EB
The SpiNXT Fecal DNA Extraction Kit is based on silica membrane based spin column technology, specifically optimized for fecal samples containing high levels of PCR inhibitors such as bile salts, polysaccharides, and complex organic compounds. Stool samples are homogenized and subjected to optimized lysis conditions to ensure efficient disruption of microbial and host cells. Released DNA selectively binds to the silica membrane, while proteins, salts, and inhibitory substances are effectively removed through sequential washing steps. Purified genomic DNA is eluted in a low-salt buffer or nuclease-free water, yielding high-purity DNA suitable for sensitive downstream molecular applications. The method eliminates the need for phenol/chloroform extraction and supports rapid, reproducible processing of multiple samples.
| Format: | Mini spin column |
| Technology: | Silica membrane |
| Sample Type: | Stool Sample |
| Yield: | Depends upon the quality & quantity of the starting material used |
| Target: | Genomic DNA, Fecal DNA |
| A260/280: | 1.6–1.9 |
| Available Pack Sizes: | 50 T, 250 T |
| Throughput Compatibility: | Manual |
| Commercial Name | Cat No. | Pack Size |
|---|---|---|
| SpiNXT Fecal DNA Extraction Kit | G2MBR4-0650 | 50 Rxn |
| SpiNXT Fecal DNA Extraction Kit | G2MBR4-0651 | 250 Rxn |
Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.
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