SpiNXT Fecal DNA Extraction kits

SpiNXT Fecal DNA Extraction kits

The SpiNXT Fecal DNA Extraction Kit is a robust and reliable solution for the isolation of high-quality genomic DNA from fresh or frozen stool (fecal) samples. The kit is optimized to efficiently recover DNA from complex fecal matrices, enabling simultaneous extraction of host DNA and microbial DNA for advanced molecular and microbiome studies. Its streamlined workflow ensures consistent performance while minimizing inhibitor carryover commonly associated with fecal samples.

Kit components: Buffer FLB-1 | Buffer FLB-2 | Buffer FLB-3 | Binding Buffer | Buffer TE | Proteinase K | Proteinase Dissolving Buffer | Wash Buffer 1 | Buffer EB

Blood DNA Extraction

Overview


The SpiNXT Fecal DNA Extraction Kit is based on silica membrane based spin column technology, specifically optimized for fecal samples containing high levels of PCR inhibitors such as bile salts, polysaccharides, and complex organic compounds. Stool samples are homogenized and subjected to optimized lysis conditions to ensure efficient disruption of microbial and host cells. Released DNA selectively binds to the silica membrane, while proteins, salts, and inhibitory substances are effectively removed through sequential washing steps. Purified genomic DNA is eluted in a low-salt buffer or nuclease-free water, yielding high-purity DNA suitable for sensitive downstream molecular applications. The method eliminates the need for phenol/chloroform extraction and supports rapid, reproducible processing of multiple samples.

Product Features


Blood DNA Extraction Kits
  • Binding Capacity: 20-30µg genomic DNA
  • Recommended Input Amount: 100-150mg of stool sample
  • Elution Volume: ≥ 30 µl
  • Purity: A260/280 - 1.8±0.1, A260/230 - 2.0±0.1
  • Expected Yield: Depends upon the quality & quantity of the starting material used

Applications


Gut Microbiome Analysis
  • Extraction of microbial DNA for community profiling and diversity studies.
Pathogen Detection & Identification
  • Detection of bacterial, viral, and parasitic DNA from fecal specimens.
PCR & Quantitative PCR (qPCR)
  • High-quality DNA suitable for routine amplification assays.
  • Reliable DNA input for pathogen load quantification and microbial abundance studies.
Restriction Fragment Length Polymorphism (RFLP)
  • Genetic variation analysis following restriction enzyme digestion.
  • Simultaneous analysis of host and microbial genetic material.
16S rRNA Gene Analysis
  • Ideal for bacterial taxonomy and microbiota composition studies.
Sequencing Applications (Sanger & NGS)
  • DNA compatible with library preparation for targeted or metagenomic sequencing.

Panel Specifications


Format: Mini spin column
Technology: Silica membrane
Sample Type: Stool Sample
Yield: Depends upon the quality & quantity of the starting material used
Target: Genomic DNA, Fecal DNA
A260/280: 1.6–1.9
Available Pack Sizes: 50 T, 250 T
Throughput Compatibility: Manual

Workflow / How It Works


Extraction workflow

Data Analysis and Reporting


Fecal DNA Extraction
Fecal DNA Extraction
Data/Report
Data/Report

Ordering Information


Commercial Name Cat No. Pack Size
SpiNXT Fecal DNA Extraction Kit G2MBR4-0650 50 Rxn
SpiNXT Fecal DNA Extraction Kit G2MBR4-0651 250 Rxn

Product Enquiry Form


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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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