MagNXT Plasmid DNA Extraction Kit

MagNXT Plasmid DNA Extraction Kit

The MagNXT Plasmid DNA Extraction Kit is designed for the rapid and efficient extraction of plasmid DNA from bacterial cells. The kit employs magnetic bead-based purification technology to ensure high quality DNA with consistent yield and purity. The extracted plasmid DNA is suitable for routine molecular biology and genetic research applications.

Kit components: MagPure Particles | Buffer A | Buffer B | Buffer C | RNaseA | Buffer BPW1 | Buffer BPW2 | Buffer EB

Blood DNA Extraction

Overview

The MagNXT Plasmid DNA Extraction Kit utilizes magnetic bead based purification technology for efficient isolation of plasmid DNA from bacterial cells. The kit employs optimized buffers and paramagnetic beads to enable effective cell lysis, selective DNA binding, and removal of contaminants. Plasmid DNA binds specifically to magnetic beads and is separated using a magnetic field, eliminating the need for centrifugation. After washing, high-purity plasmid DNA is eluted using a low-salt buffer, making it suitable for downstream molecular biology applications. The workflow supports both manual and automated extraction platforms.

Product Features

Blood DNA Extraction Kits
  • Recommended Input Amount: 1-5 ml bacterial culture
  • Binding Mechanism: Super paramagnetic Beads
  • Elution Volume: ≥ 30 µl
  • Purity: A260/280 -1.8±0.1
  • Expected Yield: Upto 32μg (depends upon culture volume, growth conditions, host strain and plasmid copy number)

Applications

Transformation

  • Purified plasmid DNA can be directly used for efficient transformation into competent bacterial cells.

Transfection

  • The high purity of the extracted DNA supports reliable transfection in mammalian and other eukaryotic cell lines.

PCR

  • The DNA serves as a high-quality template for conventional and real-time PCR assays.

DNA Sequencing

  • The extracted plasmid DNA is suitable for Sanger and automated DNA sequencing applications.

Restriction Enzyme Digestion

  • Compatible with conventional restriction enzyme digestion for cloning and plasmid analysis.

Panel Specifications

Format: Paramagnetic Beads
Technology: Magnetic bead
Sample Type: Bacterial Culture
Yield: Upto 32μg
Target: Plasmid DNA
A260/280: 1.6 - 1.9
Available Pack Sizes: 50 / 250 T
Throughput Compatibility: Manual

Workflow / How It Works

Extraction workflow

Data Analysis and Reporting

Plasmid DNA Extraction
DNA Bioanalyzer

Ordering Information

Commercial Name Cat No. Pack Size
MagNXT Plasmid DNA Extraction Kit G2MBR4-0611 50 T
MagNXT Plasmid DNA Extraction Kit G2MBR4-0611 250 T

Product Enquiry Form

Resources to get started

Product Brochure
IFU (Instructions for Use)
Data File

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Since its inception in 2016, Genes2me has been constantly striving towards setting a benchmark in the diagnostics space by introducing premium quality (Made in India) diagnostic kits which are CE-IVD, ISO-13485:2016, and ISO 9001:2015 certified, assuring our clients of unparalleled quality and compliance with international standards.

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