LeoNext Solutions for NGS Library Prep

Illumina

For quick and accurate large-scale sequencing, Illumina's sequencing method utilizes clonal array construction and its own reversible terminator technology. A wide range of applications in genomics, transcriptomics and epigenomics are made possible by novel and adaptable sequencing systems.

DNA Sequencing

NGS technology can sequence the DNA of any organism, delivering useful information in response to nearly any scientific issue. DNA sequencing is a highly scalable technique that can be applied to small, targeted regions or the complete genome using a variety of approaches allowing researchers to examine and better understand health and disease.

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DNA Library preparation kits-

Universal DNA Library Preparation kit
NGS3101-01	LeoNext Universal DNA Library Prep Kit for Illumina® V3	24 rxn
NGS3101-02	LeoNext Universal DNA Library Prep Kit for Illumina® V3	96 rxn
NGS3102-03	LeoNext Universal DN Library Prep Kit for Illumina® V3 (PCR-Free)	24 rxn
NGS3102-04	LeoNext Universal DNA Library Prep Kit for Illumina® V3 (PCR-Free)	96 rxn
DNA Library Preparation Kits for Enzymatic Fragmentation
NGS3104-01	LeoNext Universal Plus DNA Library Prep Kit for Illumina®	24 rxn
NGS3104-02	LeoNext Universal Plus DNA Library Prep Kit for Illumina®	96 rxn
FFPE DNA Library Preparation Kit
NGS3105-01	LeoNext Universal Pro DNA Library Prep Kit for Illumina®	24 rxn
NGS3105-02	LeoNext Universal Pro DNA Library Prep Kit for Illumina®	96 rxn

DNA Adapters-

Single-Indexed Adapters
NGS3108-01	LeoNext DNA Adapters Set1 for Illumina®	10 µl each
NGS3108-02	LeoNext DNA Adapters Set1 for Illumina®	40 µl each
NGS3109-01	LeoNext DNA Adapters Set 2 for Illumina®	10 µl each
NGS3109-02	LeoNext DNA Adapters Set 2 for Illumina®	40 µl each
NGS3110	LeoNext DNA Adapters Set 3 for Illumina®	20 µl each
NGS3111	LeoNext DNA Adapters Set 4 for Illumina®	20 µl each
NGS3112	LeoNext DNA Adapters Set 5 for Illumina®	20 µl each
NGS3113	LeoNext DNA Adapters Set 6 for Illumina®	20 µl each
Dual-Indexed Adapter
NGS3114	LeoNext Multiplex Oligos Set 4 for Illumina®	192 rxn
NGS3115	LeoNext Multiplex Oligos Set 5 for Illumina®	192 rxn
UMI Adapters
NGS3116	LeoNext Dual Index UMI DNA Adapters Set 1 for Illumina®	20 µl each
NGS3117	LeoNext Dual Index UMI DNA Adapters Set 2 for Illumina®	20 µl each
NGS3118	LeoNext Dual Index UMI DNA Adapters Set 3 for Illumina®	20 µl each
NGS3119	LeoNext Dual Index UMI DNA Adapters Set 4 for Illumina®	20 µl each

Amplicon Sequencing

Amplicon Library Preparation Kits
NGS3120-01	LeoNext AmpSeq Library Prep Kit V2	24 rxn
NGS3120-02	LeoNext AmpSeq Library Prep Kit V2	96 rxn
NGS3121-01	LeoNext AmpSeq Library Prep Kit V3	24 rxn
NGS3121-02	LeoNext AmpSeq Library Prep Kit V3	96 rxn

Amplicon Library Preparation Adapter
NGS3122-01	LeoNext AmpSeq Adapters 1 -12 for Illumina®	12 ×10 rxn
NGS3122-02	LeoNext AmpSeq Adapters 13 -24 for Illumina®	12 ×10 rxn
NGS3122-03	LLeoNext AmpSeq Adapters 25 - 48 for Illumina®	24 ×10 rxn
NGS3122-04	LeoNext AmpSeq Adapters 49 -72 for Illumina®	24 ×10 rxn
NGS3122-05	LeoNext AmpSeq Adapters 73 -96 for Illumina®	24 ×10 rxn

DNA Clean Beads

DNA Clean-up & Size-Selection
NGS3194-01	LeoNext DNA Clean Beads	5 ml
NGS3194-02	LeoNext DNA Clean Beads	60 ml
NGS3194-03	LeoNext DNA Clean Beads	450 ml

Quantification Kits -

Library Quantification Kits
NGS3196	LeoNext Library Quantification Kit for Illumina®	500 rxn
NGS3197	LeoNext Library Quantification Kit for Illumina®	500 rxn
NGS3198	LeoNext Library Quantification Kit for Illumina®	500 rxn
NGS3199	LeoNext Library Quantification Kit for Illumina®	500 rxn
NGS3200	DNA Standard 1-6	8 rxn
NGS3201	Library Dilution Buffer	50 ml
LeoNext Assay Kits
NGS3202-01	LeoNext dsDNA HS Assay Kit	100 assays
NGS3202-02	LeoNext dsDNA HS Assay Kit	500 assays
NGS3203-01	LeoNext 1x dsDNA HS Assay Kit	100 assays
NGS3203-02	LeoNext 1x dsDNA HS Assay Kit	500 assays

RNA Sequencing

RNA sequencing (RNA-Seq) is redefining transcriptome research. RNA-Seq enables researchers to detect both known and unknown characteristics in a single test, allowing them to identify transcript isoforms, gene fusions, single nucleotide variations, and other traits without regard for past information.

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RNA Library Preparation Kits

NGS3168-01	LeoNext Universal V6 RNA-Seq Library Prep Kit for Illumina®	24 rxn
NGS3168-02	LeoNext Universal V6 RNA-Seq Library Prep Kit for Illumina®	96 rxn

RNA Adapters

Single-Indexed Adapters
NGS3170-01	LeoNext RNA Adapters Set 1 for Illumina®	10 μl each
NGS3170-02	LeoNext RNA Adapters Set 1 for Illumina®	40 μl each
NGS3171-01	LeoNext RNA Adapters Set 2 for Illumina®	10 μl each
NGS3171-02	LeoNext RNA Adapters Set 2 for Illumina®	40 μl each
NGS3172	LeoNext RNA Adapters Set 3 for Illumina®	20 μl each
NGS3173	LeoNext RNA Adapters Set 4 for Illumina®	20 μl each
NGS3174	LeoNext RNA Adapters Set 5 for Illumina®	20 μl each
NGS3175	LeoNext RNA Adapters Set Set 6 for Illumina®	20 μl each
Dual-Indexed Adapters
NGS3176	LeoNext RNA Multiple Oligos Set 1 for Illumina®	192 rxn each
NGS3177	LeoNext RNA Multiplex Oligos Set 2 for Illumina®	192 rxn each

RNA Clean Beads

RNA Clean-up
NGS3195-01	LeoNext RNA Clean Beads	5 ml
NGS3195-02	LeoNext RNA Clean Beads	40 ml
NGS3195-03	LeoNext RNA Clean Beads	450 ml

RNA Quantification Kits

LeoNext Assay Kits
NGS3204-01	LeoNext RNA HS Assay Kit	100 assays
NGS3204-02	LeoNext RNA HS Assay Kit	500 assays
NGS3205-01	LeoNext RNA BR Assay Kit	100 assays
NGS3205-02	LeoNext RNA BR Assay Kit	500 assays

Ion Torrent

Ion Torrent next generation sequencing technology, powered by semiconductor chips, enables you to implement a quick and easy workflow that can be used for a wide variety of applications, such as inherited diseases, oncology, Infectious diseases, reproductive genomics, human identification, agronomy, and more. Research stretches to meet your needs.

DNA Sequencing

NGS technology can sequence the DNA of any organism, delivering useful information in response to nearly any scientific issue. DNA sequencing is a highly scalable technique that can be applied to small, targeted regions or the complete genome using a variety of approaches allowing researchers to examine and better understand health and disease.

View Details

DNA Library preparation kits

Universal DNA Library Preparation Kits
NGS3136-01	LeoNext Universal DNA Library Prep Kit for Ion Torrent®	24 rxn
NGS3136-02	LeoNext Universal DNA Library Prep Kit for Ion Torrent®	96 rxn

DNA Adapters

Amplicon Library Preparation Adapters
NGS3139-01	LeoNext AmpSeq Adapters 1 - 12 for Ion Torrent®	12 ×10 rxn
NGS3139-02	LeoNext AmpSeq Adapters 13 - 24 for Ion Torrent®	12 ×10 rxn
NGS3140-03	LeoNext AmpSeq Adapters 25 - 48 for Ion Torrent®	24 ×10 rxn
NGS3140-04	LeoNext AmpSeq Adapters 49 - 72 for Ion Torrent®	24 ×10 rxn
NGS3140-05	LeoNext AmpSeq Adapters 73 - 96 for Ion Torrent®	24 ×10 rxn

MGI

DNA Sequencing

NGS technology can sequence the DNA of any organism, delivering useful information in response to nearly any scientific issue. DNA sequencing is a highly scalable technique that can be applied to small, targeted regions or the complete genome using a variety of approaches allowing researchers to examine and better understand health and disease.

View Details

DNA Library preparation Kits

Universal DNA Library Prepration Kits
NGS3142-01	LeoNext Universal DNA Library Prep Kit for MGI®	24 rxn
NGS3142-02	LeoNext Universal DNA Library Prep Kit for MGI®	96 rxn
DNA Library Preparation Kits for Enzymatic Fragmentation
NGS3144-01	LeoNext Universal Plus DNA Library Prep Kit for MGI®	24 rxn
NGS3144-02	LeoNext Universal Plus DNA Library Prep Kit for MGI®	96 rxn
FFPE DNA Library preparation kits
NGS3145-01	LeoNext Universal Pro DNA Library Prep Kit for MGI®	24 rxn
NGS3145-02	LeoNext Universal Pro DNA Library Prep Kit for MGI®	96 rxn

DNA Adapters

Dual-Indexed UMI Adapters
NGS3146-01	LeoNext DNA Adapters Set 8 for MGI®	10 μl each
NGS3146-02	LeoNext DNA Adapters Set 8 for MGI®	40 μl each

Amplification Module

Library preparation kit
NGS3150-01	LeoNext AmpSeq General Library Prep Kit for MGI®	24 rxns
NGS3150-02	LeoNext AmpSeq General Library Prep Kit for MGI®	96 rxns

RNA Sequencing

RNA sequencing (RNA-Seq) is redefining transcriptome research. RNA-Seq enables researchers to detect both known and unknown characteristics in a single test, allowing them to identify transcript isoforms, gene fusions, single nucleotide variations, and other traits without regard for past information.

View Details

RNA Library Prep (for MGI®)

Ultra Fast & Universal RNA Library Preparation Kits
NGS3183-01	LeoNext Universal V6 RNA-Seq Library Prep Kit for MGI®	24 rxn
NGS3183-02	LeoNext Universal V6 RNA-Seq Library Prep Kit for MGI®	96 rxn
Single-Indexe Adapters
NGS3185-01	LeoNext RNA Adapters Set 8 for MGI®	10 μl each
NGS3185-02	LeoNext RNA Adapters Set 8 for MGI®	40 μl each

Others

A typical NGS library preparation workflow has multiple steps, several of which involve enzymes such as Extracting and purifying DNA (or RNA) from a sample, Fragmenting DNA, Fragment end-repair, Ligating adaptors, Amplifying the library, Quantitating and pooling for sequencing etc.

FFPE Repair
NGS3152-01	LeoNext DNA Damage Repair Kit	24 rxn
NGS3152-02	LeoNext DNA Damage Repair Kit	96 rxn
Enzymatic Fragmentation Module
NGS3153-01	LeoNext Universal Plus Fragmentation Module	24 rxn
NGS3153-02	LeoNext Universal Plus Fragmentation Module	96 rxn
Enzymes for DNA Library Preparation
TDP-2000U	T4 DNA Polymerase	2,000 U
TPK-10000U	T4 Polynucleotide Kinase	10,000 U
TDL02	Rapi T4 DNA Ligase	600,000 U
DPK01	DNA pol I Klenow fragment	5,000 U
DPK02	DNA pol I Klenow fragment exonuclease	5,000 U
PDP-01	Phi29 DNA Ploymerase	250 U
PDP-02	Phi29 DNA Ploymerase	1,250 U
Amplification Module
NGS3206-01	Fantom Uc Super-Fidelity DNA Polymerase for Library Amplification	100 U
NGS3206-02	Fantom Uc Super-Fidelity DNA Polymerase for Library Amplification	500 U
Modules for RNA Library Preparation
mRNA Capture Beads
NGS3186-01	LeoNext mRNA Capture Beads	24 rxn
NGS3186-02	LeoNext mRNA Capture Beads	96 rxn
RNA Fragmentation
NGS3187-01	LeoNext 2 × Frag / Prime Buffer	96 rxn
rRNA Depletion Kits
NGS3188-01	LeoNext rRNA Depletion Kit (Human / Mouse / Rat)	24 rxn
NGS3188-02	LeoNext rRNA Depletion Kit (Human / Mouse / Rat)	96 rxn
NGS3189-01	LeoNext rRNA Depletion Kit (Bacteria)	12 rxn
NGS3189-02	LeoNext rRNA Depletion Kit (Bacteria)	24 rxn
NGS3190-01	LeoNext rRNA Depletion Kit (Plant)	24 rxn
NGS3190-02	LeoNext rRNA Depletion Kit (Plant)	96 rxn